In this study, genotype independent in planta transformation of
cotton (Gossypium hirsutum) variety G.Cot.10, using injection
method and cut method has been reported. Agrobacterium tumefaciens
strain containing construct pBIN1F carrying the nptII and cry1F
gene was used for plant transformation. The integration of the gene
was confirmed by using PCR. The transformation experiment was
performed by optimizing two important parameters: age of the
seedlings and co-cultivation duration with Agrobacterium. When the
effect of the age of the seedlings and co-cultivation duration
evaluated three days old seedlings and co-cultivated for three days
yielded higher survival percentage as well the frequency of
transformation in both methods (injection method, i.e. 6% and cut
method, i.e. 4%). Overall higher frequency of transformation was
yielded in injection method i.e. 1.16% while in cut method overall
frequency of transformation obtained was 0.83%.
General
Imprint: |
Lap Lambert Academic Publishing
|
Country of origin: |
Germany |
Release date: |
February 2012 |
First published: |
February 2012 |
Authors: |
Vanrajsinh Solanki
• Vikas Khandelwal
|
Dimensions: |
229 x 152 x 7mm (L x W x T) |
Format: |
Paperback - Trade
|
Pages: |
108 |
ISBN-13: |
978-3-8484-0468-1 |
Categories: |
Books
|
LSN: |
3-8484-0468-0 |
Barcode: |
9783848404681 |
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