The objective of this study was to understand the pitfalls of the
poor oocyte cleavage and subsequent embryo production by assessing
the developmental competence of the oocytes in buffalo using
parthenogenesis as a model which was compared with IVF (natural
activation). Buffalo ovaries were collected from slaughter house
and matured oocytes were subjected to either IVF (control) or
chemical activation (treatment) 7% ethanol (ET)for 7 min ] 2.5 mM
6-Di methyl amino purine (6-DMAP) for 4 hr, or 7% ET for 7 min + 10
ug/ml Cycloheximide (CHX) for 6 hr or 7% ET for 7 min + 2.5 mM
6-DMAP + 10 ug/ml CHX for 6 hr]. Fertilized and chemically
activated oocytes were cultured in mSOF medium for 8 days to study
embryo development.In conclusion, the results of parthenogenesis,
revealed that buffalo oocytes had better inherent developmental
competence and the poor cleavage and embryo development following
IVF, in our study, may partly be due to the poor quality of
frozen-thawed sperm, improper sperm capacitation and/ or
fertilization. Further, high ambient temperature adversely affected
the cleavage and embryo development following IVF.
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