This second edition provides new and updated methods on the principles underlying modern protein analysis, from statistical issues to gel-based and mass spectrometry-based applications. Chapters detail protein quantification as basis for realisation of quantitative studies, gel-based and mass spectrometry-based quantification techniques, TMT, IPTL, PRM, MALDI Imaging, SILAC, PTM analysis, DIA, cross-linking, and the up-to-date topics of software and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Quantitative Methods in Proteomics, Second Edition aims to provide comprehensive and competent overview in the important and still growing field of quantitative proteomics.

Through the rapid development of proteomics methods and technologies, an enormous amount of data was created, leading to a wide-spread rethinking of strategy design and data interpretation. In Data Mining in Proteomics: From Standards to Applications, experts in the field present these new insights within the proteomics community, taking the historical evolution as well as the most important international standardization projects into account. Along with basic and sophisticated overviews of proteomics technologies, standard data formats, and databases, the volume features chapters on data interpretation strategies including statistics, spectra interpretation, and analysis environments as well as specialized tasks such as data annotation, peak picking, phosphoproteomics, spectrum libraries, LC/MS imaging, and splice isoforms. As a part of the highly successful Methods in Molecular Biology (TM) series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and cutting-edge, Data Mining in Proteomics: From Standards to Applications is a well-balanced compendium for beginners and experts, offering a broad scope of data mining topics but always focusing on the current state-of-the-art and beyond.

Do the antitrust laws have a place in the digital economy or are they obsolete? That is the question raised by the government's legal action against Microsoft, and it is the question this volume is designed to answer. America's antitrust laws were born out of the Industrial Revolution. Opponents of the antitrust laws argue that whatever merit the antitrust laws may have had in the past they have no place in a digital economy. Rapid innovation makes the accumulation of market power practically impossible. Markets change too quickly for antitrust actions to keep up. And antitrust remedies are inevitably regulatory and hence threaten to `regulate business'. A different view - and, generally, the view presented in this volume - is that antitrust law can and does have an important and constructive role to play in the digital economy. The software business is new, it is complex, and it is rapidly moving. Analysis of market definition, contestibility and potential competition, the role of innovation, network externalities, cost structures and marketing channels present challenges for academics, policymakers and judges alike. Evaluating consumer harm is problematic. Distinguishing between illegal conduct and brutal - but legitimate - competition is often difficult. Is antitrust analysis up to the challenge? This volume suggests that antitrust analysis `still works'. In stark contrast to the political rhetoric that has surrounded much of the debate over the Microsoft case, the articles presented here suggest neither that Microsoft is inherently bad, nor that it deserves a de facto exemption from the antitrust laws. Instead, they offer insights - for policymakers, courts, practitioners, professors and students of antitrust policy everywhere - on how antitrust analysis can be applied to the business of making and marketing computer software.

Communications markets have made much progress towards competition and deregulation in recent years. However, it is increasingly clear, in the age of the Internet and the digital revolution, that much more needs to be done, and that new approaches, both at the Federal Communications Commission and in Congress, will be required to complete the task. In this volume, the Progress and Freedom Foundation presents nine papers by communications policy experts and government policymakers that show how to finish the job of deregulating communications markets and reforming the FCC. The Telecommunications Act of 1996 was a landmark piece of legislation for an industry moving from a monopoly orientation towards competition, but additional steps are needed to complete the process of implementing the pro-competitive, deregulatory vision of the act. Bringing together a group of the caliber represented in this book makes possible the best recommendations about the exact nature of those necessary changes. In this volume, the most difficult and politically-charged hot-button issues involving local and long distance competition, universal service, spectrum allocation, program content regulation, and the public interest doctrine are confronted head-on. As importantly, the authors recommend specific reform proposals to be considered by the Federal Communications Commission and Congress. The ideas contained in the experts' essays were presented and debated at a conference hosted by The Progress & Freedom Foundation, which was held in Washington, DC, on December 8, 2000. The Progress & Freedom Foundation studies the impact of the digital revolution and its implications for public policy. It conducts research in fields such as electronic commerce, telecommunications and the impact of the Internet on government, society and economic growth. It also studies issues such as the need to reform government regulation, especially in technology-intensive fields such as medical innovation, energy and environmental regulation.

Through the rapid development of proteomics methods and technologies, an enormous amount of data was created, leading to a wide-spread rethinking of strategy design and data interpretation. In Data Mining in Proteomics: From Standards to Applications, experts in the field present these new insights within the proteomics community, taking the historical evolution as well as the most important international standardization projects into account. Along with basic and sophisticated overviews of proteomics technologies, standard data formats, and databases, the volume features chapters on data interpretation strategies including statistics, spectra interpretation, and analysis environments as well as specialized tasks such as data annotation, peak picking, phosphoproteomics, spectrum libraries, LC/MS imaging, and splice isoforms. As a part of the highly successful Methods in Molecular Biology(TM) series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Authoritative and cutting-edge, Data Mining in Proteomics: From Standards to Applications is a well-balanced compendium for beginners and experts, offering a broad scope of data mining topics but always focusing on the current state-of-the-art and beyond.

Communications markets have made much progress towards competition and deregulation in recent years. However, it is increasingly clear, in the age of the Internet and the digital revolution, that much more needs to be done, and that new approaches, both at the Federal Communications Commission and in Congress, will be required to complete the task. In this volume, the Progress and Freedom Foundation presents nine papers by communications policy experts and government policymakers that show how to finish the job of deregulating communications markets and reforming the FCC. The Telecommunications Act of 1996 was a landmark piece of legislation for an industry moving from a monopoly orientation towards competition, but additional steps are needed to complete the process of implementing the pro-competitive, deregulatory vision of the act. Bringing together a group of the caliber represented in this book makes possible the best recommendations about the exact nature of those necessary changes. In this volume, the most difficult and politically-charged hot-button issues involving local and long distance competition, universal service, spectrum allocation, program content regulation, and the public interest doctrine are confronted head-on. As importantly, the authors recommend specific reform proposals to be considered by the Federal Communications Commission and Congress. The ideas contained in the experts' essays were presented and debated at a conference hosted by The Progress & Freedom Foundation, which was held in Washington, DC, on December 8, 2000. The Progress & Freedom Foundation studies the impact of the digital revolution and its implications for public policy. It conducts research in fields such as electronic commerce, telecommunications and the impact of the Internet on government, society and economic growth. It also studies issues such as the need to reform government regulation, especially in technology-intensive fields such as medical innovation, energy and environmental regulation.

Do the antitrust laws have a place in the digital economy or are they obsolete? That is the question raised by the government's legal action against Microsoft, and it is the question this volume is designed to answer. America's antitrust laws were born out of the Industrial Revolution. Opponents of the antitrust laws argue that whatever merit the antitrust laws may have had in the past they have no place in a digital economy. Rapid innovation makes the accumulation of market power practically impossible. Markets change too quickly for antitrust actions to keep up. And antitrust remedies are inevitably regulatory and hence threaten to `regulate business'. A different view - and, generally, the view presented in this volume - is that antitrust law can and does have an important and constructive role to play in the digital economy. The software business is new, it is complex, and it is rapidly moving. Analysis of market definition, contestibility and potential competition, the role of innovation, network externalities, cost structures and marketing channels present challenges for academics, policymakers and judges alike. Evaluating consumer harm is problematic. Distinguishing between illegal conduct and brutal - but legitimate - competition is often difficult. Is antitrust analysis up to the challenge? This volume suggests that antitrust analysis `still works'. In stark contrast to the political rhetoric that has surrounded much of the debate over the Microsoft case, the articles presented here suggest neither that Microsoft is inherently bad, nor that it deserves a de facto exemption from the antitrust laws. Instead, they offer insights - for policymakers, courts, practitioners, professors and students of antitrust policy everywhere - on how antitrust analysis can be applied to the business of making and marketing computer software.

This second edition provides new and updated methods on the principles underlying modern protein analysis, from statistical issues to gel-based and mass spectrometry-based applications. Chapters detail protein quantification as basis for realisation of quantitative studies, gel-based and mass spectrometry-based quantification techniques, TMT, IPTL, PRM, MALDI Imaging, SILAC, PTM analysis, DIA, cross-linking, and the up-to-date topics of software and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Quantitative Methods in Proteomics, Second Edition aims to provide comprehensive and competent overview in the important and still growing field of quantitative proteomics.

Through a variety of primary sources--including speeches, poems, magazine articles, and book excerpts--this collection illustrates the origins, ambitions, and political legacy of the American Progressivism movement (1886-1924). A general introduction offers a history of the movement and a brief discussion of recent historiographical debates; headnotes introduce each selection and provide historical and political context.

Vorliegende Arbeit schliesst an den Untersuchungen von Kunze (1969) uber die Papilla filiformis des Menschen an, in der aufgrund der Anwesenheit und Art der Nervenendformationen gezeigt werden konnte, dass sie vornehmlich der Rezeption der Tastempfindung dient. Diese Arbeit beschreibt die Morphologie der als pilzfoermigen Zungenpapille bezeichneten Papilla fungiformis von Ratte, Meerschweinchen, Nutria und Wasserschwein in lichtoptischer, ultrastruktureller und rasterelektronenmikro- skopischer Sicht. Die vergleichende Darstellung der Ausformung der Papille, der Art der Epithel- und Bindegewebsbeziehung, der Modus der Gefass- und Nerven- versorgung soll der Klarung der Fragen dienen, ob einerseits bei nahe verwandten Formen ein einheitliches Bauprinzip fur die Papilla fungiformis vorliegt, und ob andererseits mit der Zunahme der Koerpergroesse (Vergleich zwischen Meerschwein- chen und Wasserschwein, dem groessten rezenten Nager) Proportionsverschie- bungen in der Papillenarchitektur einhergehen. Die anatomisch-physiologischen Betrachtungen uber die Warme- und Kalteempfindungen der Zunge von Rein (1925) und Strughold (1925) sollen mit der Art und Lokalisation der Nerven- endkoerperehen innerhalb der Bindegewebspapille bei den untersuchten Formen in Beziehung gesetzt, die Funktion der Papilla fungiformis als Organ der Zungen- oberflache spezifizieren. Material und Methode Die Papilla fungiformis der Zungenspitze und des vorderen Zungendrittels von Ratte (4oe) (Rattus norvegicus albino Wistar), Meerschweinchen (2 Cjl) (Cavia aperea f. porcellus) und Nutria (2 ) (Myocastor coypus) wurden untersucht. Um die Morphologie der Papille auf oe ihre Artspezifitat und Groessenbeziehung hin zu uberprufen, wurde als der nachste Verwandte des Meerschweinchens und als groesster rezenter Nager das Wasserschwein (1 Cjl) (Hydrochoerus capybara) als Vergleichstier herangezogen. Licht- und Elektronenmikroskopie.

Urspriinglich verfolgte diese Arbeit nur die Absicht, die in meinem 1973 als Band 1 dieser Schriftenreihe verijffentlichten Buch "Die Steuerbelastung der Untemehmung" in ihren Grundziigen dargel te Teilsteuerrechnung zu dynamisieren und dadurch ihren Anwendungsbereich auf mehrperiodige Kal kiile auszudehnen. 1m Laufe der Untersuchungen zeigte sich jedoch bald, daB die dabei auftretenden rechnerischen Grundsatzpl'obleme einer theoretischen Basis bedurften, um zu abgesicherten Er.gebnissen zu gelangen. Aus diesem Grunde hat Manfred Eisenach seine Darlegungen in den Rahmen eines Bei trages zur entscheidungsorientierten Steuerplanung gestellt. Die Analyse des Beziehungszusammenhangs zwischen dynamischer Steuerbelastungsrechnung und steuerlicher Planung ergab, daB eine als Entscheidungshilfe konzipierte prospektiv-dynamische Teilsteuerrechnung mit (quantifizierender) Steuer planung identisch ist; die damit gegebenen Moglichkeiten sind ganz offen sichtlich von groBer praktischer Relevanz. Die Zukunftsbezogenheit der Planung bedingt ein weites theoretisches Unter suchungsfeld mit einer Vielfalt von Verankerungspunkten. In erster Linie dadurch erkliirt sich der Umfang des Buches. Die Ausfiihrungen konnten sich nur teilweise auf Voruntersuchungen stiitzen; wegen des neuartigen Unter suchungsansatzes muBten viele Probleme auch von Grund auf neu analysiert werden."

Jedem Ingenieur, der auf dem Gebiete der Antriebstedmik arbeitet, sind aus seiner Berufsarbeit die Masdllnenelemente gelaufig. Leider sind ihm aber in vielen Fallen gewisse, damit im Zusammenhang stehende widttige wirtsdtaft- lidte Tatbestande nicht bekannt. Sie kommen in den folgenden Zahlen zum Ausdruck: Unter den Erzeugnisgruppen des gesamten Masdtinenbaues nehmen die An- triebselemente - zu ihnen gehoeren Gleit- und Walzlager, Kupplungen, Zahn- rader und andere Transmissionsteile - sowie die Zahnradgetriebe und die stufenlos regelbaren Getriebe einen beachtlidten Platz ein. Umsatzmassig reprasentierten sie im Jahre 1952 einen Wert von etwa 700 Millionen DM und standen damit in der fadtlidten Unterteilung an vierter Stelle. Der Anteil der direkten Ausfuhr in diesen Erzeugnissen betrug etwa 10 Prozent der vor- genannten Summe. In der Gruppe Stahlgelenkketten wurde im Jahre 1952 ein Gesamtumsatzwert von rund 33 Millionen DM bei einem Ausfuhranteil von 25 bis 30 Prozent erstellt. - Im gleidten Zeitabsdtnitt beliefen sich die Gesamtumsatze der Her- steller von Lederriemen auf etwa 20 Millionen DM. Hier betrugen die Export- erloese etwa 10 Prozent dieser Summe. - Die Kautsdtukindustrie beziffert ihre Gesamtumsatze in Gummikeilriemen auf etwa 19 Millionen DM fur das Jahr 1952, dabei lag der Wert der direkten Ausfuhr bei etwa 15 Prozent. - In Textil- und Kunststoffflachriemen wurden sdtatzungsweise noch groessere Um- satze erzielt.

This is a reproduction of a book published before 1923. This book may have occasional imperfections such as missing or blurred pages, poor pictures, errant marks, etc. that were either part of the original artifact, or were introduced by the scanning process. We believe this work is culturally important, and despite the imperfections, have elected to bring it back into print as part of our continuing commitment to the preservation of printed works worldwide. We appreciate your understanding of the imperfections in the preservation process, and hope you enjoy this valuable book. ++++ The below data was compiled from various identification fields in the bibliographic record of this title. This data is provided as an additional tool in helping to ensure edition identification: ++++ Staatshandbuch F�r Das Gro�herzogtum Sachsen Sachsen-Weimar-Eisenach B�hlau, 1823 History; General; History / General

This is a reproduction of a book published before 1923. This book may have occasional imperfections such as missing or blurred pages, poor pictures, errant marks, etc. that were either part of the original artifact, or were introduced by the scanning process. We believe this work is culturally important, and despite the imperfections, have elected to bring it back into print as part of our continuing commitment to the preservation of printed works worldwide. We appreciate your understanding of the imperfections in the preservation process, and hope you enjoy this valuable book. ++++ The below data was compiled from various identification fields in the bibliographic record of this title. This data is provided as an additional tool in helping to ensure edition identification: ++++ Werke Homerus, Johann Jacob Bodmer

Two Volumes In One. This scarce antiquarian book is included in our special Legacy Reprint Series. In the interest of creating a more extensive selection of rare historical book reprints, we have chosen to reproduce this title even though it may possibly have occasional imperfections such as missing and blurred pages, missing text, poor pictures, markings, dark backgrounds and other reproduction issues beyond our control. Because this work is culturally important, we have made it available as a part of our commitment to protecting, preserving and promoting the world's literature.