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Out of 1500 nasopharyngeal swab samples collected from patients who
were admitted to different maternity clinics in Dhaka city, 111
optochin resistant strains were isolated. As a confirmatory test of
Streptococcus pneumoniae, i.e., Bile solubility was checked. A
total of 37 optochin resistant bile soluble isolates were obtained,
designated as optochin resistant bile soluble S. pneumoniae as
other - hemolytic streptococci are not bile soluble. For further
identification, bile soluble isolates were subjected to PCR of the
lytA gene specific for S. pneumonia an 32 (87%) isolates were found
to be lytA positive. Thus, it can be suggested that for
confirmatory identification of S. pneumoniae, bile solubility test
together with PCR of lytA gene should be carried out in routine
microbilogical investigation. Antimicrobial susceptibility test
revealed multiple drug resistance among the organisms. About 64.86%
were resistant to more than three agents (multi drug resistant).
Higher MIC values were obtained for azithromycin ( 256 microg/ml),
erythromycin ( 256 microg/ml) and ciprofloxacin ( 8 microg/ml)
considering the CLSI break point.
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