Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (Phytophthora and Botrytis) and mammalian infection (Penicillium marneffei and Candida). Genetic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits.

In the past ten years there has been a rapid growth of the research and application area known as Lab-on-a-Chip. After an initial focus on electrokinetic separation techniques on chip, the scope of the field has widened to include topics like microfluidics, DNA analysis, cell analysis, microreactors and mass spectrometer interfacing. As well as the analytical chemistry community, synthetic chemists, chemical engineers, biochemists and biomedical engineers are now also becoming more and more interested in using new micro- and nanotechnological techniques.
This first Lab-on-a-Chip book contains a broad collection of papers on microtechnology, microfluidics, analytical methods and applications. All contributions are written by leading researchers in their respective fields, and provide new scientists with an overview of the field, to make him/her aware of the enormous opportunities offered by modern technology. The work presented in this book will definitely stimulate readers to new ideas and concepts, and lead to further innovations in this area.
- Provides a quick introduction into the different aspects of this field
- Describes technology that has already revolutionized the world of chemical and biochemical analysis and synthesis
- All contributions are written by leading researchers in their respective fields

The use of silicon-based microsystems for chemical analysis is one of the most promising concepts in the recent developments in micro system technology (MST). It is expected that chemical sensors will be increasingly integrated in so-called miniaturized total analysis systems (uTAS), a concept first presented by Ciba-Geigy. In such systems, all steps in a chemical determination, from sampling to detection and data treatment, are integrated in one miniature instrument. uTAS offer a variety of advantages over conventional analysis systems such as improved analytical performance, reduced reagent and power consumption, small size, possibility of new and more complicated functions, higher reliability and lower fabrication costs. Application of uTAS may be found in fields like process industry, environmental monitoring, medical diagnostics, aeronautics, automotive industry, and more.

Fungi are an economic very important class of microbes. Not only do they host a range of versatile enzymes used in industrial applications (biofuels, laundry, food processing), as well do they produce several very important pharmaceutical drugs (statins and penicillins). Moreover, fungal pathogens can cause great damage in agricultural production (Phytophthora and Botrytis) and during mammalian infections (Penicillium marneffei and Candida). Transformation of DNA is used to understand the genetic basis behind these traits. Several different techniques have been developed over the years and readily shown to be decisive methods to improve fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples to be used in laboratories worldwide.

Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (Phytophthora and Botrytis) and mammalian infection (Penicillium marneffei and Candida). Genetic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits.

Fungi are an economic very important class of microbes. Not only do they host a range of versatile enzymes used in industrial applications (biofuels, laundry, food processing), as well do they produce several very important pharmaceutical drugs (statins and penicillins). Moreover, fungal pathogens can cause great damage in agricultural production (Phytophthora and Botrytis) and during mammalian infections (Penicillium marneffei and Candida). Transformation of DNA is used to understand the genetic basis behind these traits. Several different techniques have been developed over the years and readily shown to be decisive methods to improve fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples to be used in laboratories worldwide.

In two dozen short biographies of John Calvin's friends including some who turned into enemies Machiel A. van den Berg paints an intimate portrait of the great Reformer's life and circle that most of us have never seen. / In these pages we accompany Calvin from his early boyhood in Noyon to his student days in Paris and Orleans, to his pastorate in and exile from Geneva, all the way to his deathbed. We meet his famous Reformer friends, such as Martin Bucer, William Farel, Heinrich Bullinger, Theodore Beza, and John Knox, but also friends whose names are more obscure: his cousin Pierre Robert Olivtan, the first translator of the Bible into French; Ren de France of French royalty; Laurent de Normandie, the mayor of Noyon who later escaped to Geneva; Pierre Viret, his best friend of all; and Idelette van Buren, his beloved wife during their brief but blissful marriage. / Calvin may be known as a scholar who preferred his study to imperial and ecclesiastical politics, but he was also a rebel of faith against the papacy, which controlled most of Europe and had a price on the heads of all "reform-minded" citizens, especially their leaders. Allegiances to Rome were constantly in flux in the empires of Europe as were the lives and welfare of dissenters and Calvins life was full of perilous risks, political intrigue, much harried travel, and many relocations. / Peppered with quotations from Calvin's voluminous letters, Friends of Calvin abounds with secret court relationships, love affairs, death threats, poisonings, and narrow midnight escapes from the pursuing authorities showing a full-blooded and dangerous side of the bookish Reformer's life. Readers of these colorful narratives will come to see how much Calvins friends influenced his life and thought.