A reliable system for expression and purification of the recombinant Cyt2Aa1 toxin has been developed. The recombinant Cyt2Aa1 toxin has been produced, characterized, followed by the construction of the cysteine mutants V186C and L189C by site directed mutagenesis.The hemolytic activity of the V186C mutant exceeds that of wild type Cyt2Aa1 toxin and of the L189C mutant.Calcein release assay experiments have been done to examine the activity of the toxin with different artificial liposomes. It was found that Cyt2Aa1 toxin is very active with DMPC, DMPC+DMPG unilamellar liposomes.

Demand for electrical energy continues to grow steadily, and is particularly strong in those countries on the threshold of industrialization. Electricity grid upgrades can not keep pace with the growing energy demand due to economical and environmental considerations that electric utilities are facing today to extend their power systems. Due to this situation, operators are looking for ways to utilize the existing power systems more efficiently. Consequently, most of power systems, nowadays, are working with heavy loading conditions. These new highly stressed operating conditions of these power systems arises different forms of power system instability. This book looks at stressed modern power system together with integrated wind resources and the role of FACTS devices in enhancing voltage stability.

This book looks at the modern electrical energy meter reading. It investigates the application for power line carrier as the communication medium between the meters and the power system. Electric meters are the building blocks of the future smart network applications. Moreover, it illustrates the use of meters as a means for load control which is a necessary aspect for modern Demand Side management. The proposed concepts have been experimentally verified over a sample low voltage distribution network.

El lector debe pensar que lo fantastico se presenta como fuese un atentado contra la realidad que lo circunscribe. El narrador reconoce y hace un planteamiento de lo imposible como fuese una voluntad realista de los narradores fantasticos. Lo fantastico es como el producto de una retorica especifica que construye una maquinaria textual que permite la irrupcion de lo innombrable en el mundo asi representado. Lo fantastico es un Simbolo que incluye la experiencia intuitiva mediante la realidad del autor. Intentamos en esa obra solucionar los problemas de confusion de algunos criticos para teorizar lo fantastico."