In this study the role of epicuticular wax in transmission of CLCuV has been studied. Epicuticular waxes plays very important role in hindering the attachment of insects to the leaves. In this study the wax deficient mutant plants GaWM3 were used as experimental plants while Gossypium arboreum (786) were used as negatively controlled plants as it is reported that whitefly can not transmit virus in it and G. hirsutum (MNH-93) were taken as negative and positive control plants because it has severe viral attack in past. It is important to mention here that GaWM3 were produced in our laboratory at CEMB from Gossypium arboreum (786) with the only difference of having 50% less wax than Gossypium arboreum (786). It was found that the G. hirsutum (MNH-93) was CLCuV positive and G. arboreum (786) was CLCuV negative. But most strikingly the wax deficient GaWM3 mutant plants which contain 50% less than G. arboreum (786) wax were CLCuV positive while the plants from which these mutants were made (G. arboreum,786) were CLCuV negative. If wax act act a barrier than, the damage of CLCuV on cotton can be overcome by by increasing the wax content.

Biodegradation of paranitrophenol (PNP) was carried out using Pseudomonas pseudomallei capable for PNP degradation. For enhanced biodegradation this strain was subjected to UV ray, chemical and gamma ray mutagenesis. UV and chemical mutagenesis was not fruitful as no PNP hyper degrading bacterial strain was obtained. Gamma ray mutagenesis was positive for PNP degradation and hyper degrading bacterial strain was obtained. Both parent and mutant in presence of glucose, efficiently degraded PNP. When concentration of glucose increased from 50 to 100 ppm then 50 ppm PNP was degraded within 12 hours by mutant and in 20 hours by parent. So glucose had positive effect on PNP degradation and resulted in enhanced PNP degradation. Effect of phenol was also checked on PNP degradation. The result showed that immobilization of the bacterial culture was best practice for PNP degradation. Similarly addition of glucose helped enhanced PNP degradation and ammonium sulphate provided good nitrogen source. After this toxicity of treated and untreated PNP was also checked by exposing to fish, that showed that after degradation of PNP no toxic metabolites were produced.

Global demand of energy is ever increasing and its resources due to extensive exploitation arc fastly diminishing. The days of inexpensive fossil energy are clearly numbered, the credibility of nuclear energy has fallen to new low, and fusion energy stands decades of more for practical realization. It is therefore imperative to turn to renewable raw materials for energy. At present there is worldwide interest in the process of converting photo synthetically derived substances into food. Chemicals and fuels. The imminent storage of fossil fuel has intensified the research for bioconversion of lignocelluosic material to fuels. The availability of solar energy needs to be linked up with cellulosic and hemicelluloses productivity. -glucosidase is one of the component enzymes of the cellulase complex and is widely distributed in nature. It catalyzes the hydrolysis of arly and alkyl glycosides as well as of cellobiose to glucose which is inhibitory to the endo and exo cellulases during cellulose hydrolysis. This book explain the production of Beta Glucosidase and its characterization.