This book constitutes the refereed proceedings of the 10th International Workshop on Biomedical Image Registration, WBIR 2020, which was supposed to be held in Munich, Germany, in July 2022.The 11 full and poster papers together with 17 short papers included in this volume were carefully reviewed and selected from 32 submitted papers. The papers are organized in the following topical sections: optimization, deep learning architectures, neuroimaging, diffeomorphisms, uncertainty, topology and metrics.