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Bacterial genomics is a mature research interdisciplinary field,
which is approached by ecologists, geneticists, bacteriologists,
molecular biologists and evolutionary biologists working in
medical, industrial and basic science. Thanks to the large
diffusion of bacterial genome analysis, Bacterial Pangenomics:
Methods and Protocols is able to provide the most recent
methodologies about the study of bacterial pangenomes by covering
the three major areas: the experimental methods for approaching
bacterial pangenomics, the bio informatic pipelines for analysis
and annotation of sequence data and finally the methods for
inferring functional and evolutionary features from the pangenome.
Written in the successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step,
readily reproducible protocols and notes on troubleshooting and
avoiding known pitfalls. Authoritative and easily accessible,
Bacterial Pangenomics: Methods and Protocols will serve as a field
guide for both qualified bacterial genomics investigators who want
to update their technical knowledge, for less experienced
researchers who want to start working with bacterial genomics and
pangenomics, as well as serving as a manual and supplemental
textbook for graduate students of genomics and bioinformatics.
This completely revised edition explores novel discoveries in
bacterial genomic research, with a focus on technical and
computational improvements as well as methods used for bacterial
pangenome analysis, which relies on microbiome studies and
metagenomic data. Beginning with up-to-date sequencing methods, the
book continues with sections covering methods for deep phylogenetic
analysis, the role of metagenomic data in understanding the
genomics of the many yet uncultured bacteria, progress in
genome-to-phenome inference, as well as computational genomic
tools. Written for the highly successful Methods in Molecular
Biology series, chapters include the type of practical detail
necessary for reproducible results in the lab. Authoritative and
up-to-date, Bacterial Pangenomics: Methods and Protocols, Second
Edition serves as an ideal guide for both highly qualified
investigators in bacterial genomics and for less experienced
researchers, including students and teachers, who could use a
reference for approaching genomic analysis and genome data.
This completely revised edition explores novel discoveries in
bacterial genomic research, with a focus on technical and
computational improvements as well as methods used for bacterial
pangenome analysis, which relies on microbiome studies and
metagenomic data. Beginning with up-to-date sequencing methods, the
book continues with sections covering methods for deep phylogenetic
analysis, the role of metagenomic data in understanding the
genomics of the many yet uncultured bacteria, progress in
genome-to-phenome inference, as well as computational genomic
tools. Written for the highly successful Methods in Molecular
Biology series, chapters include the type of practical detail
necessary for reproducible results in the lab. Authoritative and
up-to-date, Bacterial Pangenomics: Methods and Protocols, Second
Edition serves as an ideal guide for both highly qualified
investigators in bacterial genomics and for less experienced
researchers, including students and teachers, who could use a
reference for approaching genomic analysis and genome data.
Bacterial genomics is a mature research interdisciplinary field,
which is approached by ecologists, geneticists, bacteriologists,
molecular biologists and evolutionary biologists working in
medical, industrial and basic science. Thanks to the large
diffusion of bacterial genome analysis, Bacterial Pangenomics:
Methods and Protocols is able to provide the most recent
methodologies about the study of bacterial pangenomes by covering
the three major areas: the experimental methods for approaching
bacterial pangenomics, the bio informatic pipelines for analysis
and annotation of sequence data and finally the methods for
inferring functional and evolutionary features from the pangenome.
Written in the successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step,
readily reproducible protocols and notes on troubleshooting and
avoiding known pitfalls. Authoritative and easily accessible,
Bacterial Pangenomics: Methods and Protocols will serve as a field
guide for both qualified bacterial genomics investigators who want
to update their technical knowledge, for less experienced
researchers who want to start working with bacterial genomics and
pangenomics, as well as serving as a manual and supplemental
textbook for graduate students of genomics and bioinformatics.
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