Handbook of Tractography presents methods and applications of MR diffusion tractography, providing deep insights into the theory and implementation of existing tractography techniques and offering practical advice on how to apply diffusion tractography to research projects and clinical applications. Starting from the design of MR acquisition protocols optimized for tractography, the book follows a pipeline approach to explain the main methods behind diffusion modelling and tractography, including advanced analysis of tractography data and connectomics. An extensive section of the book is devoted to the description of tractography applications in research and clinical settings to give a complete picture of tractography practice today. By focusing on technology, models and applications, this handbook will be an indispensable reference for researchers and students with backgrounds in computer science, mathematics, physics, neuroscience and medical science.