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This detailed volume provides methods to guide assay development,
procedures designed to investigate the chemokine and
glycosaminoglycan (GAG) networks, as well as their interactions, in
a wide range of organs and tissues in disease and in health. The
initial chapters in this book present in vivo models used to
examine the roles of chemokines and GAGs in normal physiology and
in the pathophysiology of disease. The book then explores present
cell- and tissue-based in vitro assays to examine chemokine:GAG
interactions. Finally, analytic approaches are presented that
provide assays for measuring GAGs, chemokines, and cellular
responses. Written for the highly successful Methods in Molecular
Biology series, chapters include introductions to their respective
topics, lists of the necessary materials and reagents, step-by-step
and readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls. Authoritative and
practical, Chemokine-Glycosaminoglycan Interactions: Methods and
Protocols serves as an ideal guide for researchers seeking to
analyze chemokine and GAG functions, interactions, and molecular
mechanisms in vivo and in vitro.
This detailed volume explores methods and techniques reflecting how
viruses can be a beneficial force for all levels of life as well as
the mammalian microbiota. Beginning with methods for the
development and study of viruses, the book continues with current
techniques and approaches used to develop new treatments from
virus-derived vaccines to vectors and proteins, such as a range of
models and methods designed to measure therapeutic efficacy of
virus-derived biologics and therapeutic proteins. Written for the
highly successful Methods in Molecular Biology series, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Viruses as
Therapeutics: Methods and Protocols is an ideal guide for
researchers working to further understand how viruses can benefit
lifeforms and even prevent damage from other viruses.
This detailed volume explores methods and techniques reflecting how
viruses can be a beneficial force for all levels of life as well as
the mammalian microbiota. Beginning with methods for the
development and study of viruses, the book continues with current
techniques and approaches used to develop new treatments from
virus-derived vaccines to vectors and proteins, such as a range of
models and methods designed to measure therapeutic efficacy of
virus-derived biologics and therapeutic proteins. Written for the
highly successful Methods in Molecular Biology series, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Viruses as
Therapeutics: Methods and Protocols is an ideal guide for
researchers working to further understand how viruses can benefit
lifeforms and even prevent damage from other viruses.
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