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This study was conducted to investigate the influence of ACC
deaminase rhizobacteria on the ACC-induced classical "triple"
response in etiolated pea seedlings. Etiolated pea seedlings were
exposed to different concentrations of ACC (0, 2, through 10 mmol
L-1), in 100 ml glass beakers placed in airtight mason jars wrapped
in green foil and incubated under dark for seven days. In another
study pea seedlings were inoculated with five strains of
rhizobacteria which vary in their ACC-deaminase activity. These
inoculated pea seedlings were exposed to 10 mmol L-1 ACC and
incubated in the darkness at 25 3 C. Results revealed that
exogenous application of ACC had a concentration-dependent effect
in creating classical "triple" response in etiolated pea seedlings.
Our study concludes that the inoculation with rhizobacteria
containing ACC deaminase could be used to decrease ACC which
produces due to a variety of biotic and abiotic stresses in plants.
Oyster mushroom (Pleurotus ostreatus) is an important and is a good
source of proteins, vitamins and minerals. In Pakistan local
strains of mushroom are cultivated on various agricultural
by-products as substrate. The widely used substrate for cultivation
of the Oyster mushroom in Asia is rice straw. Keeping in view, its
importance as a good source of energetic product having high
protein value, the present studies were conducted to investigate
the yield of exotic strains on cotton waste alone and were compared
with amended rice husk under our natural environmental conditions.
It was found that cotton waste + rice substrate gave the maximum
yield of edible mushroom with the strain P. (sajor-caju)
pulmonarious (WC-537) as compared to the substrate applied alone.
Salinity is one of the major constraints, whichhamper agricultural
production. The higherconcentration of salts in root zone may
stimulateendogenous ethylene biosynthesis in roots,
whichconsequently inhibits its growth. One of the majormechanism
utilized by plant growth promotingrhizobacteria (PGPR) to
facilitate plant growth anddevelopment is the lowering of ethylene
levels in theplant roots by deamination
of1-aminocyclopropane-1-carboxylic acid (ACC), theimmediate
precursor of ethylene in plants. In thegiven study, three
pre-isolated bacterial strainshaving ACC deaminase activity were
used forinoculation of wheat seeds. Inoculated seeds weresown in
salt affected field, fertilized with NPK@120-100-60 kg ha-1,
respectively. Data regardingvarious growth parameters and yield
parameters wasrecorded at the time of harvesting and
statisticallyanalyzed. From data it was concluded that plantgrowth
promoting rhizobacteria containingACC-deaminase activity can
enhance the growth andyield of wheat under salinity stress field
conditionsand the strain ACC10 performs the best.
A pot experiment was conducted to study the comparative
effectiveness of rhizobacteria containing either ACC-deaminase
and/or nitrogen fixing activity for promoting growth of tomato
(Lycopersicon esculantum Mill) in pot trial. Isolation of
rhizobacteria containing ACC- deaminase and/or nitrogen fixing
ability was done by dilution plate technique. Characterization of
selected isolates like root colonization, ACC-deaminase activity
and indole acetic acid production was done. Seeds of tomato were
inoculated with broth of selected isolates. Hoagland nutrient
solution was used to provide nutrition. The results showed that
rhizobacteria containing both ACC-deaminase and nitrogen fixing
activity were more effective than rhizobacteria containing either
ACC-deaminase or nitrogen fixing activity alone for growth
promotion of tomato under controlled condition.
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