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This second edition provides new and updated protocols to
investigate clock-controlled parameters including period
estimations and entrainment measurements and modelling. Chapters
detail promoter activity using luciferase reporters,
fluorescent-tagging of clock proteins and cellular imaging, protein
level measurements, regulated RNA splicing detection,
post-translational modifications, protein-protein interactions, and
protein DNA/chromatin interactions. Additional chapters focus on
seasonal processes like hypocotyl growth and flowering time control
in annuals and trees, and techniques on soybean. Written in the
format of the highly successful Methods in Molecular Biology
series, each chapter includes an introduction to the topic, lists
necessary materials and reagents, includes tips on troubleshooting
and known pitfalls, and step-by-step, readily reproducible
protocols. Authoritative and practical, Plant Circadian Networks:
Methods and Protocols, Second Edition is designed not only for the
chronobiology community dealing with circadian biology but also for
the plant community in general. "Period estimation and rhythm
detection in timeseries data using BioDare2, the free, online,
community resource" is available open access under a Creative
Commons Attribution 4.0 International License via
link.springer.com.
This second edition provides new and updated protocols to
investigate clock-controlled parameters including period
estimations and entrainment measurements and modelling. Chapters
detail promoter activity using luciferase reporters,
fluorescent-tagging of clock proteins and cellular imaging, protein
level measurements, regulated RNA splicing detection,
post-translational modifications, protein-protein interactions, and
protein DNA/chromatin interactions. Additional chapters focus on
seasonal processes like hypocotyl growth and flowering time control
in annuals and trees, and techniques on soybean. Written in the
format of the highly successful Methods in Molecular Biology
series, each chapter includes an introduction to the topic, lists
necessary materials and reagents, includes tips on troubleshooting
and known pitfalls, and step-by-step, readily reproducible
protocols. Authoritative and practical, Plant Circadian Networks:
Methods and Protocols, Second Edition is designed not only for the
chronobiology community dealing with circadian biology but also for
the plant community in general. "Period estimation and rhythm
detection in timeseries data using BioDare2, the free, online,
community resource" is available open access under a Creative
Commons Attribution 4.0 International License via
link.springer.com.
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