This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Comprehensive and up-to-date, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols, Second Edition aims to arm stem cell biologists, both novice and expert, with invaluable protocols that are currently being used in various laboratories around the world.

This volume captures the rapid developments in the field of induced pluripotent stem (iPS) cells, which have provided novel opportunities and approaches both for better understanding a number of human diseases and for developing new platforms for drug development and screening for such diseases. Specifically, representative protocols on various disease models have been collected from labs around the world. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization is an ideal reference for scientists working on furthering iPS research.

This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols, Second Edition aims to arm stem cell biologists, both novice and expert, with invaluable protocols that are currently being used in various laboratories around the world.

A Chinese Taoist book about meditation, this book was first translated by Richard Wilhelm (also translator, in the 1920s, of the Chinese philosophical classic the I Ching). Wilhelm, was German, and his translations from Chinese to German were later translated to English by Cary F. Baynes. According to Wilhelm, LuDongbin was the main originator of the material presented in the book suggests that the material is from Quanzhen School founder Wang Chongyang, a student of Lu Dongbin). More recently (2013), the same work has been translated by Pei Yeing and published by Ancient Wisdom Publications. Since the original (now, public domain) work is a Chinese translation from German, it is strongly suggested that this work should be considered as an alternative resource for any serious practitioner. The translator of this work is a lifetime practitioner of meditation is currently living in Shanghai, China but a Malaysian Chinese by birth.

The Best of James Allen. Four books in one -Including: As a Man Thinketh * The Path of Prosperity * Above Life's Turmoil * The Way of Peace. (Plus a James Allen short biography by the editor/complier)This collection shows how is no other facet of the Spiritual Path that is more liberating and empowering than the understanding how the quality of our thoughts can guide and form our destiny and the quality of life we lead. We can contemplate about God, Love and Oneness, but unless we learn early that first and foremost our thoughts that guide and lead us, other ideals and pursuits might be unfruitful. This collection shows that all the creative powers and self-determination is our cosmic inheritance, our birthright. It is all knowable.

It is said that many intelligent and otherwise capable people owned the Secret Doctrine but only a handful actually understood and comprehended it in its totality. The problem is that the esteemed author, Madame Blavatsky used a prose and style that many contemporary readers would have trouble reading and understanding. The sheer size of the books is formidable. In addition the Secret Doctrine attempts to cover everything from the complete Cosmology of The Universe, God and the devil - just to name a few of the subjects. The third, also called Lost Volume of the work was published and mostly written by her disciple Annie Bessant, who naturally knew a great deal less than her Master. According to P.G.B. Bowen, Blavatsky gave the following instructions regarding the study of the Secret Doctrine: "Reading the Secret .Doctrine page by page as one reads any other book (she says) will only end us in confusion. The first thing to do, even if it takes years, is to get some grasp of the "Three Fundamental Principles" given in the Proem. Follow that up by study of the Recapitulation - the numbered items in the Summing Up to Vol. I (Part 1.) Then take the Preliminary Notes (Vol. II) and the Conclusion (Vol. II)." Reading this guide book and appendix A and B will certainly help understanding the most important book in the last two hundred years.