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From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis. Coverage extends the potential of realtime as well as advocates simplifications of the probe technologies. It also presents a new simplified molecular beacon design, EasyBeacons, and demonstrates the utility in DNA methylation profiling.
From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis. Coverage extends the potential of realtime as well as advocates simplifications of the probe technologies. It also presents a new simplified molecular beacon design, EasyBeacons, and demonstrates the utility in DNA methylation profiling.
Wer ist der Autor der seltsamen E-Mail? Was bezweckt er? - Thomas Arcus steht vor einem Ratsel. Der unbekannte Absender scheint ihn genau zu kennen. Aber die Worte ergeben keinen Sinn. Gemeinsam mit Freunden versucht Arcus Klarheit zu gewinnen. In Gedanken reist er in die eigene Vergangenheit, durchlebt noch einmal Schlusselmomente seiner Jugend. Andeutungen werden aufgedeckt, aber die Sache bleibt ratselhaft. Er erhalt weitere ungewohnliche E-Mails. Dann eine Einladung auf die Ile de Re an die Atlantikkuste Frankreichs. Die Fahrt zum Atlantik wird zu einer Reise surrealer Begegnungen. Auf der Ile de Re angekommen, macht sich Arcus erfolglos auf die Suche nach dem unbekannten Absender. Statt dessen lernt er die junge Celine kennen ...
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