With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed. Human Cytomegaloviruses: Methods and Protocols is designed to be an inclusive document covering all of the techniques and approaches necessary to understand and study the pathobiology of human cytomegalovirus. Topics covered include the history of human cytomegaloviruses, techniques to culture and grow the virus in model cell types, the use of primary cells for the study of human cytomegalovirus pathogenesis, modern molecular techniques for assessing the biological consequences of viral infection, animal models for study of cytomegalovirus replication and numerous other topics of current interest. Written in the successful Methods in Molecular Biology series format, each chapter includes an introduction, list of necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Human Cytomegaloviruses: Methods and Protocols serves as a tool for basic scientists as well as clinical scientists with an interest in the basic fundamental aspects of viral gene expression and specific aspects of viral pathogenesis.

This new edition explores and provides an update on the biology and pathogenesis of human cytomegalovirus infection. Modern techniques that are currently being utilized to investigate the molecular aspects of viral infection, as well as how these new research studies are leading to new approaches to mitigate disease, are also provided. Given the key role the virus plays in significant acute and chronic human disease in all stages of life, from newborns to seniors, the need for clear methodologies to further explore the biology of HCMV infection and mitigation strategies is readily apparent. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Human Cytomegaloviruses: Methods and Protocols, Second Edition serves as an ideal scientific reference for basic and clinical scientists and medical personnel on the modern understanding of the pathobiology of the virus, and the approaches, techniques, and models to study human cytomegalovirus infection and disease.

This new edition explores and provides an update on the biology and pathogenesis of human cytomegalovirus infection. Modern techniques that are currently being utilized to investigate the molecular aspects of viral infection, as well as how these new research studies are leading to new approaches to mitigate disease, are also provided. Given the key role the virus plays in significant acute and chronic human disease in all stages of life, from newborns to seniors, the need for clear methodologies to further explore the biology of HCMV infection and mitigation strategies is readily apparent. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Human Cytomegaloviruses: Methods and Protocols, Second Edition serves as an ideal scientific reference for basic and clinical scientists and medical personnel on the modern understanding of the pathobiology of the virus, and the approaches, techniques, and models to study human cytomegalovirus infection and disease.

With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed. Human Cytomegaloviruses: Methods and Protocols is designed to be an inclusive document covering all of the techniques and approaches necessary to understand and study the pathobiology of human cytomegalovirus. Topics covered include the history of human cytomegaloviruses, techniques to culture and grow the virus in model cell types, the use of primary cells for the study of human cytomegalovirus pathogenesis, modern molecular techniques for assessing the biological consequences of viral infection, animal models for study of cytomegalovirus replication and numerous other topics of current interest. Written in the successful Methods in Molecular Biology series format, each chapter includes an introduction, list of necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Human Cytomegaloviruses: Methods and Protocols serves as a tool for basic scientists as well as clinical scientists with an interest in the basic fundamental aspects of viral gene expression and specific aspects of viral pathogenesis.