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This new edition explores and provides an update on the biology and
pathogenesis of human cytomegalovirus infection. Modern techniques
that are currently being utilized to investigate the molecular
aspects of viral infection, as well as how these new research
studies are leading to new approaches to mitigate disease, are also
provided. Given the key role the virus plays in significant acute
and chronic human disease in all stages of life, from newborns to
seniors, the need for clear methodologies to further explore the
biology of HCMV infection and mitigation strategies is readily
apparent. Written in the highly successful Methods in Molecular
Biology format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
up-to-date, Human Cytomegaloviruses: Methods and Protocols, Second
Edition serves as an ideal scientific reference for basic and
clinical scientists and medical personnel on the modern
understanding of the pathobiology of the virus, and the approaches,
techniques, and models to study human cytomegalovirus infection and
disease.
With the significant medical role that human cytomegalovirus plays
in human disease (from acute disease following primary infection,
to chronic disease due to life-long viral persistence), the need to
better understand human cytomegalovirus biology and pathogenesis is
needed. Human Cytomegaloviruses: Methods and Protocols is designed
to be an inclusive document covering all of the techniques and
approaches necessary to understand and study the pathobiology of
human cytomegalovirus. Topics covered include the history of human
cytomegaloviruses, techniques to culture and grow the virus in
model cell types, the use of primary cells for the study of human
cytomegalovirus pathogenesis, modern molecular techniques for
assessing the biological consequences of viral infection, animal
models for study of cytomegalovirus replication and numerous other
topics of current interest. Written in the successful Methods in
Molecular Biology series format, each chapter includes an
introduction, list of necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls. Authoritative and
easily accessible, Human Cytomegaloviruses: Methods and Protocols
serves as a tool for basic scientists as well as clinical
scientists with an interest in the basic fundamental aspects of
viral gene expression and specific aspects of viral pathogenesis.
With the significant medical role that human cytomegalovirus plays
in human disease (from acute disease following primary infection,
to chronic disease due to life-long viral persistence), the need to
better understand human cytomegalovirus biology and pathogenesis is
needed. Human Cytomegaloviruses: Methods and Protocols is designed
to be an inclusive document covering all of the techniques and
approaches necessary to understand and study the pathobiology of
human cytomegalovirus. Topics covered include the history of human
cytomegaloviruses, techniques to culture and grow the virus in
model cell types, the use of primary cells for the study of human
cytomegalovirus pathogenesis, modern molecular techniques for
assessing the biological consequences of viral infection, animal
models for study of cytomegalovirus replication and numerous other
topics of current interest. Written in the successful Methods in
Molecular Biology series format, each chapter includes an
introduction, list of necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls. Authoritative and
easily accessible, Human Cytomegaloviruses: Methods and Protocols
serves as a tool for basic scientists as well as clinical
scientists with an interest in the basic fundamental aspects of
viral gene expression and specific aspects of viral pathogenesis.
This new edition explores and provides an update on the biology and
pathogenesis of human cytomegalovirus infection. Modern techniques
that are currently being utilized to investigate the molecular
aspects of viral infection, as well as how these new research
studies are leading to new approaches to mitigate disease, are also
provided. Given the key role the virus plays in significant acute
and chronic human disease in all stages of life, from newborns to
seniors, the need for clear methodologies to further explore the
biology of HCMV infection and mitigation strategies is readily
apparent. Written in the highly successful Methods in Molecular
Biology format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
up-to-date, Human Cytomegaloviruses: Methods and Protocols, Second
Edition serves as an ideal scientific reference for basic and
clinical scientists and medical personnel on the modern
understanding of the pathobiology of the virus, and the approaches,
techniques, and models to study human cytomegalovirus infection and
disease.
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