This volume provides state-of-the-art techniques for studying various aspects of cholesterol homeostasis, including its uptake, synthesis and efflux from the cell, as well as its trafficking within the cell. Chapters also cover techniques for studying the regulation of cholesterol homeostasis at both the transcriptional and post-translational levels, as well as studying the membrane topology and structure of cholesterol-related proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cholesterol Homeostasis: Methods and Protocols aims to provide key techniques in tackling the investigation of cholesterol homeostasis.

This volume provides state-of-the-art techniques for studying various aspects of cholesterol homeostasis, including its uptake, synthesis and efflux from the cell, as well as its trafficking within the cell. Chapters also cover techniques for studying the regulation of cholesterol homeostasis at both the transcriptional and post-translational levels, as well as studying the membrane topology and structure of cholesterol-related proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cholesterol Homeostasis: Methods and Protocols aims to provide key techniques in tackling the investigation of cholesterol homeostasis.

It is the tail-end of the first millennium, and there is something very rotten in the heart of Jorvik. A mad, Saxon earl perches precariously atop the throne of the old Viking king, barely controlling a court of predatory schemers hellbent on tearing whatever strips of power they can from the dying corpse of the city. Yet there is something far, far worse than Machiavellian politicians stalking the back alleys and byways of the once great capital of the north. Persuaded out of his self-imposed exile to find a missing bridegroom, Olin Odinson is thrown into a sinister world of cannibals, Satanists, demons, and witches. Yet, as he struggles to come to terms with the altered nature of the city he once called home, it is the unavoidable meeting with his abandoned sister that fills Olin's heart with dread. Languishing in the British Royal Armouries, Olin's sword reminisces about the good old days, spinning an anachronistic adventure mystery, that effortlessly blends old and new with startling results.

The explosive conclusion of A Crow Among Ravens "Strapped to Little Olin's back, I can feel the beating of his heart through my scabbard. He's excited. Ready for the fray. Primed for action. You've no doubt heard of the calm before the storm? Well, ever one to buck against tradition, this is the storm before The Calm." Can the Krau-Y-Kree and his rag-tag band of ne'er do wells really take down the whole might of the mythic Red Skulls? Either way, it's gonna be beautifully bloody. What? I'm a sword! In my defence, this is what I was made to do.

The second book in the A Crow Among Ravens trilogy. Still reeling from Isolde's revelations at the end of Book One, Olin tries to juggle commitments to family and friends with his ongoing investigation into the disappearance of Snaevar Ulfson. After being summoned to the court of his old captain, the mad earl of Jorvik, Oiln begins to realise that he is being drawn into a sinister game of power politics that will decide the fate of the entire Christian world. Languishing in the British Royal Armouries, Olin's sword reminisces about the good old days, spinning an anachronistic adventure mystery, that effortlessly blends old and new with startling results.

This book is a self-help book that aims to be different than many others before it. Oftentimes, self-help books offer up an inspirational story from the author's life that seeks to motivate us. It's hard not to be motivated when reading about how someone overcame cancer, or worked their way out of extreme poverty, or overcame some form of disability. However, shortly after reading the book, the inspiration is forgotten and the reader is right back where he or she started. In order to create lasting change in a person that allows them to become autonomous, intrinsically motivated, and truly happy, you have to start with the person themselves. Livin' the Dream seeks to target the person's perspective and cause change that way. By combining classical philosophy with modern psychology research, the readers learn various perspectives and ideas on happiness which enables them to change their own perspectives. Because a person's perspective directly impacts how the person views the world, changing the reader's perspective effectively changes the world he or she lives in. To do so, the book discusses topics like choice, free will, death and loss, personality, and what is and is not under your control. Once the readers have done that, it's time for them to learn who they really are and then promptly overcome themselves and strive to always be better. This isn't a book that tells people what their dreams should be; it's a book that enables people to understand and decide for themselves what they believe their dreams should be and inspires them to live them every day.

The Odious Tale of Hulla Balloo is the dastardly story of a terrifying sea monster and his (almost) equally scary pet frog, Horace. They cast a shadow of fear over the oceans, but when Shelly, a small girl from Outer Mongolia, refuses to be a victim Balloo and Horace meet their match. Wickedly written and illustrated by Children's Bafta nominated interactive writer and director Andrew J Brown

This collection addresses current controversies about the relationship between science and religion, in which Augustine is appealed to by opposing sides, showing his continuing relevance, as well as the subtlety and complexity of his views. Questions on evolution are especially focused on, and from a variety of perspectives, often with quite different conclusions between the essays. This is truly a conversation about Augustine, science, and religion.