Microbiology is researched actively, and the field is advancing continually. It is estimated only about one percent of all of the microbe species on Earth have been studied. Although microbes were directly observed over three hundred years ago, the field of microbiology can be said to be in its infancy relative to older biological disciplines such as zoology and botany. This book will be very useful for the students of undergraduate and graduate students of pharmacy, microbiology, botany and quality control department of pharmaceutical industry. This book contains exhaustive note on current approaches related to different microscopic methods, micro-tome, sterilization, pure culture techniques and staining.