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This book describes the design, development, characterisation and
application of two novel fluorescence imaging instruments based on
spectrally resolved detector arrays (SRDAs). The simplest SRDA is
the standard colour camera, which integrates a Bayer filter array
of red, green and blue colour filters to replicate the colour
sensing capability of the human eye. The SRDAs used in this book
contain many more colours, ranging from 16 to over 100 colour
channels. Using these compact, robust and low-cost detectors for
biomedical applications opens new avenues of exploration that were
not possible before, in particular, the use of spectral imaging in
endoscopy. The work presented shows for the first time that not
only can this new type of camera be used for fluorescence imaging,
but also that it is able to resolve signals from up to 7 different
dyes - a level of multiplexing not previously achieved in tissue
with such compact and robust equipment. Furthermore, it reports the
application of a bimodal endoscope performing both reflectance and
fluorescence imaging using these cameras in an ex vivo pig
oesophagus model.
This book describes the design, development, characterisation and
application of two novel fluorescence imaging instruments based on
spectrally resolved detector arrays (SRDAs). The simplest SRDA is
the standard colour camera, which integrates a Bayer filter array
of red, green and blue colour filters to replicate the colour
sensing capability of the human eye. The SRDAs used in this book
contain many more colours, ranging from 16 to over 100 colour
channels. Using these compact, robust and low-cost detectors for
biomedical applications opens new avenues of exploration that were
not possible before, in particular, the use of spectral imaging in
endoscopy. The work presented shows for the first time that not
only can this new type of camera be used for fluorescence imaging,
but also that it is able to resolve signals from up to 7 different
dyes - a level of multiplexing not previously achieved in tissue
with such compact and robust equipment. Furthermore, it reports the
application of a bimodal endoscope performing both reflectance and
fluorescence imaging using these cameras in an ex vivo pig
oesophagus model.
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