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In recent years, molecular microbiology has emerged as a top,
cutting-edge biological discipline, thanks to the
multi-disciplinary and integrative approaches taken by
investigators seeking to understand the intricacies of the
microbial world and how it affects human health and the biosphere.
In Bacterial Cell Surfaces: Methods and Protocols, recent advances
in structural biology, proteomics, and imaging techniques, together
with the traditional biochemical and genetic approaches, are
provided in order to present an exciting look into the structure,
function, and regulation of the bacterial cell envelope. The
detailed volume contains examples of traditional and innovative
tools for the study of protein structure and function and enzymatic
activities, the purification and analysis of macromolecules and
their complexes, and the investigation of regulatory mechanisms and
cell biological processes. Written in the highly successful Methods
in Molecular Biology (TM) series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls. Authoritative and practical, Bacterial Cell
Surfaces: Methods and Protocols is aimed at the microbiologist,
biochemist, or cell biologist, whether a beginning graduate student
or a veteran experimentalist, who wishes to learn new methodologies
and take advantage of the years of research and protocol
optimization from the best laboratories around the world.
This book is dedicated to the channels and pores that belong to an
eclectic and ubiquitous class of unconventional - perhaps at times
strange - pore-forming molecules, which nevertheless play
fundamental roles in various organisms. These non-canonical
channels may take on various and sometimes complex architectures,
such as large beta-barrels or lipid-containing pores. They may
originate from bacteria, viruses or intracellular organelles. For
some of them, the physiologically relevant substrate may indeed be
ions, and for others folded polypeptides. Some are released by
cells in a soluble form that has the ability to insert into
biological membranes to exert its permeabilizing effect. Many of
these unconventional pores have been investigated by
electrophysiology, which, by its virtue of focusing on a few or
even a single unit, has provided invaluable insight into the
mechanisms and structure-function relationships of these remarkable
membrane entities. The chapters of this book highlight a
representative set of these interesting investigations.
In recent years, molecular microbiology has emerged as a top,
cutting-edge biological discipline, thanks to the
multi-disciplinary and integrative approaches taken by
investigators seeking to understand the intricacies of the
microbial world and how it affects human health and the biosphere.
In Bacterial Cell Surfaces: Methods and Protocols, recent advances
in structural biology, proteomics, and imaging techniques, together
with the traditional biochemical and genetic approaches, are
provided in order to present an exciting look into the structure,
function, and regulation of the bacterial cell envelope. The
detailed volume contains examples of traditional and innovative
tools for the study of protein structure and function and enzymatic
activities, the purification and analysis of macromolecules and
their complexes, and the investigation of regulatory mechanisms and
cell biological processes. Written in the highly successful Methods
in Molecular Biology (TM) series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls. Authoritative and practical, Bacterial Cell
Surfaces: Methods and Protocols is aimed at the microbiologist,
biochemist, or cell biologist, whether a beginning graduate student
or a veteran experimentalist, who wishes to learn new methodologies
and take advantage of the years of research and protocol
optimization from the best laboratories around the world.
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