|
Showing 1 - 2 of
2 matches in All Departments
It is now widely accepted that the extracellular matrix (ECM) is a
key determinant of tissue-specific gene expression. Signals
provided by ECM are transduced by integrins, a large and growing
superfamily of transmembrane heterodimeric cell surface receptors
that link the ECM to structural and fu- tional elements within the
cell. A wide range of cellular phenotypes have been shown to be
regulated by integrins, including growth, differentiation, mig-
tion, invasion, angiogenesis, and apoptosis. Furthermore,
abnormalities of integrin expression and function have been
implicated in the etiology of va- ous pathologic conditions,
including cardiovascular disease, inflammatory disorders, and
cancer. Thus integrins have emerged as an important class of
molecules with wide ranging implications for understanding basic
biological processes. In Integrin Protocols we provide a
wide-ranging collection of laboratory protocols intended to assist
investigators interested in integrins in working productively with
these molecules, in studying their expression, and in pot- tially
manipulating that expression to define their role(s) in relevant
biolo- cal models. Protocols are provided for the analysis of
integrin expression both at the RNA and protein levels (Chaps. 2,
5, and 7). Delcommenne and Streuli describe procedures for making
rat monoclonal antibodies specific for mouse integrins; Schneller
et al. and Arap and Huang describe methods for western blotting of
integrins and RT-PCR analysis. Protocols are included that cover
the analysis of the functional properties of integrins (Chaps. 1,
3, 4, 8, and 9 through 11). Koivunen et al.
It is now widely accepted that the extracellular matrix (ECM) is a
key determinant of tissue-specific gene expression. Signals
provided by ECM are transduced by integrins, a large and growing
superfamily of transmembrane heterodimeric cell surface receptors
that link the ECM to structural and fu- tional elements within the
cell. A wide range of cellular phenotypes have been shown to be
regulated by integrins, including growth, differentiation, mig-
tion, invasion, angiogenesis, and apoptosis. Furthermore,
abnormalities of integrin expression and function have been
implicated in the etiology of va- ous pathologic conditions,
including cardiovascular disease, inflammatory disorders, and
cancer. Thus integrins have emerged as an important class of
molecules with wide ranging implications for understanding basic
biological processes. In Integrin Protocols we provide a
wide-ranging collection of laboratory protocols intended to assist
investigators interested in integrins in working productively with
these molecules, in studying their expression, and in pot- tially
manipulating that expression to define their role(s) in relevant
biolo- cal models. Protocols are provided for the analysis of
integrin expression both at the RNA and protein levels (Chaps. 2,
5, and 7). Delcommenne and Streuli describe procedures for making
rat monoclonal antibodies specific for mouse integrins; Schneller
et al. and Arap and Huang describe methods for western blotting of
integrins and RT-PCR analysis. Protocols are included that cover
the analysis of the functional properties of integrins (Chaps. 1,
3, 4, 8, and 9 through 11). Koivunen et al.
|
|