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This second edition volume expands on the previous edition with
chapters discussing the latest developments and research
initiatives in mitochondrial functions. The chapters in this book
explore topics such as high-resolution fluorespirometry and OXPHOS
protocols in human cells, analysis of mitochondrial oxygen
consumption, mitochondrial bioenergetics, and mitochondrial
dynamics in mammalian cells. Written in the highly successful
Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls. Cutting-edge and practical, Mitochondrial
Bioenergetics: Methods and Protocols, Second Edition is a valuable
resource for students, and practitioners in the pharmaceutical
sciences, environmental sciences, and mitochondrial genetics
industries.
Increasing interest in mitochondrial bioenergetics is being driven
by the impact of drug and environmental chemical-induced
disturbances of mitochondrial function as well as hereditary
deficiencies and the progressive deterioration of bioenergetic
performance with age. These initiatives have fostered the
investigation of genetic and environmental influences on
bioenergetics. In Mitochondrial Bioenergetics: Methods and
Protocols, researchers in the field detail the practical principles
and assays designed to derive quantitative assessment of each set
of parameters that reflect different aspects of mitochondrial
bioenergetics. Written in the highly successful Methods in
Molecular Biology (TM) series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and key tips on troubleshooting and avoiding
known pitfalls Authoritative and practical, Mitochondrial
Bioenergetics: Methods and Protocols helps elevate the quality and
rate of investigative discoveries regarding disease states
associated with environmental or genetic influences on
mitochondrial bioenergetics.
Increasing interest in mitochondrial bioenergetics is being driven
by the impact of drug and environmental chemical-induced
disturbances of mitochondrial function as well as hereditary
deficiencies and the progressive deterioration of bioenergetic
performance with age. These initiatives have fostered the
investigation of genetic and environmental influences on
bioenergetics. In Mitochondrial Bioenergetics: Methods and
Protocols, researchers in the field detail the practical principles
and assays designed to derive quantitative assessment of each set
of parameters that reflect different aspects of mitochondrial
bioenergetics. Written in the highly successful Methods in
Molecular Biology (TM) series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and key tips on troubleshooting and avoiding
known pitfalls Authoritative and practical, Mitochondrial
Bioenergetics: Methods and Protocols helps elevate the quality and
rate of investigative discoveries regarding disease states
associated with environmental or genetic influences on
mitochondrial bioenergetics.
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