This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells.

Can you imagine the pressures and demands that go into the job of a Customer Service Representative (CSR) in a call centre? The incidence of job burnout among call centre CSRs is extremely high. Job Burnout is "a syndrome of emotional exhaustion, depersonalization, and reduced personal accomplishment that can occur among individuals who do 'people work' of some kind." As part of the global industry, call centres in India have experienced spectacular growth in the last few years. However, there is hardly any detailed and systematic understanding of the management practices and outcomes of call centres in India and therefore this book examines job burnout among call centres CSRs in India. A comprehensive model of job burnout is tested using two multivariate techniques- canonical correlation analysis and regression analysis; moreover, a standardized instrument to measure job burnout is also developed and validated.The book highlights the important role of social support in reducing burnout levels among CSRs and caters to professionals in the call centre industry, as well as academicians and practitioners interested in the area of job burnout.