This text covers the field of steady-state kinetics from basic principles to the control of the multi-enzyme systems which constitute metabolic pathways. Emphasis is placed on the interpretation of the kinetic behaviour of enzyme-catalyzed reactions in terms of mechanisms. Algorithms are developed which can be implemented in computer programs for the derivation of equations. The treatment of steady-state enzyme kinetics is extended to allosteric enzymes and subunit interactions in polymeric enzymes. Principles are presented which provide for mathematical analysis of the control of multi-enzyme systems. Problems are included at the end of each chapter and their solutions are found at the end of the book. This book will be a useful text for advanced undergraduates and graduate students taking courses in enzyme chemistry and enzyme kinetics.

This textbook for advanced courses in enzyme chemistry and enzyme kinetics covers the field of steady-state enzyme kinetics from the basic principles inherent in the Henri equation to the expressions that describe the control of multi-enzyme pathways. Steady-state kinetic equations are derived with the use of the connection matrix method, and an algorithm is developed that can be implemented easily for computer-based derivation of the equations. Throughout the book emphasis is placed on a proper interpretation of the kinetic behavior of multi-reactant enzymic reactions and on the interpretation of control coefficients in terms of metabolic control. Problems are included at the end of each chapter and their solutions are found at the end of the book.