This volume provides an up-to-date compilation of current methodological approaches utilized for the exploration of nucleolar structure and function. Chapters cover a diversity of protocols that include imaging of the nucleolus, analysis of ribosomal RNA transcription and processing, and genomics and proteomics of the nucleolus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Nucleolus: Methods and Protocols provides scientists with a reliable practical handbook to facilitate the investigation of this nuclear compartment at the advanced level.

Epicurus on the Self reconstructs a part of Epicurean ethics which only survives on the fragmentary papyrus rolls excavated from an ancient library in Herculaneum, On Nature XXV. The aim of this book is to contribute to a deeper understanding of Epicurus' moral psychology, ethics and of its robust epistemological framework. The book also explores how the notion of the self emerges in Epicurus' struggle to express the individual perspective of oneself in the process of one's holistic self-reflection as an individual psychophysical being.

Epicurus on the Self reconstructs a part of Epicurean ethics which only survives on the fragmentary papyrus rolls excavated from an ancient library in Herculaneum, On Nature XXV. The aim of this book is to contribute to a deeper understanding of Epicurus' moral psychology, ethics and of its robust epistemological framework. The book also explores how the notion of the self emerges in Epicurus' struggle to express the individual perspective of oneself in the process of one's holistic self-reflection as an individual psychophysical being.

This volume provides an up-to-date compilation of current methodological approaches utilized for the exploration of nucleolar structure and function. Chapters cover a diversity of protocols that include imaging of the nucleolus, analysis of ribosomal RNA transcription and processing, and genomics and proteomics of the nucleolus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Nucleolus: Methods and Protocols provides scientists with a reliable practical handbook to facilitate the investigation of this nuclear compartment at the advanced level.