Contents:
Part 1. Fundamental Aspects 1. Principles of Cryobiology Peter Mazur 2. The Water to Ice Transition. Implications for Living Cells Ken Muldrew, Jason Acker, Gloria Elliott, Locksley Mcgann Part 2. Life and Death at Low Temperatures 3. Life in the Polar Terrestrial Environment with a Focus on Algae and Cyanobacteria Josef Elster, Erica Benson 4. Microbial Life in Permafrost. Extended Times in Extreme Conditions Monica Ponder, Tatiana Vishnivetskaya, John Mcgrath and James Tiedje 5. Adaptation of Higher Plants to Freezing Roger Pearce 6. Oxidative Stress in the Frozen Plant. A Free Radical Point of View Erica Benson, David Bremner 7. Physiology, Biochemistry and Molecular Biology of Vertebrate Freeze Tolerance. The Wood Frog Kenneth B. Storey, Janet M. Storey Part 3. Freezing and Banking of Living Resources 8. Preservation of Fungi and Yeasts C. Shuhui Tan, Cor Van Ingen 9. Cryo-Conserving Algal and Plant Diversity. Historical Perspectives and Future Challenges Erica E. Benson 10. Plant Cryopreservation Akira Sakai 11. The Early History of Gamete Cryobiology Stanley P. Leibo 12. Cryobiology of Gametes and the Breeding of Domestic Animals Alban Massip, Stanley P. Leibo, Elizabeth Blesbois 13. Cryopreservation as a Supporting Measure in Species Conservation; "Not The Frozen Zoo!" Amanda R. Pickard, William V. Holt 14. Cryopreservation of Gametes and Embryos of Aquatic Species Tiantian Zhang 15. Fundamental Issues for Cell-Line Banks in Biotechnology and Regulatory Affairs Glyn Stacey Part 4. Medical Applications 16. Mechanisms of Injury Caused by In Vivo Freezing Nathan E Hoffman, John C. Bischof 17. Cryopreservation in Transfusion Medicine and Haematology Andreas Sputtek, Rebekka Sputtek 18. Cryopreservation of Human Gametes and Embryos Barry Fuller, Sharon Paynter, Paul Watson 19. The Scientific Basis for Tissue Banking Monica Wusteman Charles J. Hunt 20. Engineering Desiccation Tolerance in Mammalian Cells. Tools and Techniques Jason P. Acker, Tani Chen, Alex Fowler, Mehmet Toner 21. Stabilization of Cells During Freeze-Drying. The Trehalose Myth John H. Crowe, Lois M. Crowe, Fern Tablin, Willem Wolkers, Ann E. Oliver, Nelly M. Tsvetkova 22. Vitrification in Tissue Preservation. New Developments Mike J. Taylor, Yin C. Song and Kelvin G.M. Brockbank Part 5. The Future of Cryobiology 23. The Future of Cryobiology Nick Lane

While it is barely 50 years since the first reliable reports of the recovery of living cells frozen to cryogenic temperatures, there has been tremendous growth in the use of cryobiology in medicine, agriculture, horticulture, forestry, and the conservation of endangered or economically important species. As the first major text on cryobiology in the genomic era, Life in the Frozen State describes the current understanding of how living cells and complex organisms survive very low temperatures. Leading world experts combine fundamental theory and practice across a spectrum of species and applications to evaluate how cryobiology can benefit humanity. Chapters encompass disciplines ranging from mathematical modeling and biophysics, to the molecular biology of stress gene expression and the clinical banking of cells and tissues. This book provides a unique opportunity to explore the subject in a multidisciplinary context, which has historically been the key to realizing some of the most exciting advances in low temperature research. Features Integrates fundamental theory and practice across a broad range of species and applications Discusses cryobiology within a multidisciplinary context Emphasizes how the current knowledge of cryobiology can be applied to benefit humanity through health care and conservation