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This volume covers past and present western blot techniques, such
as diffusion blotting, slice blotting, blotting of high and low
molecular weight proteins, single cell blotting and automated
blotting. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Thorough and
cutting-edge, Western Blotting: Methods and Protocols will serve as
an invaluable reference for those interested in further study into
this fascinating field.
This volume expands upon the collection of techniques published in
Protein Electrophoresis: Methods and Protocols (2012) with more
practical and reproducible methods to study protein gel detection
and imaging. The chapters in this book cover topics such as
coomassie-brilliant blue staining of polyacrylamide gels; silver
staining techniques; microwave assisted protein staining,
de-staining, and in-solution digestion of proteins; curumin and
turmeric as an environment-friendly protein gel stain; in-gel
protein phosphotase assay using fluorogenic substrates; destaining
with fungal laccase; and radiolabeling and analysis of labeled
proteins. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Comprehensive and
practical, Protein Gel Detection and Imaging: Methods and Protocols
is a valuable resource for expert and novice scientists and
researchers who are interested in learning and experimenting with
this field.
This detailed volume provides methods and techniques for detection
after blotting. Chapters guide readers through a number of
variations on the theme of protein transfer to solid support
followed by detection, presenting adaptations of traditional
techniques, and original methods of protein blotting. Written for
the Methods in Molecular Biology series, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls.Practical and authoritative, Detection of Blotted
Proteins: Methods and Protocols presents numerous techniques based
on the Western blot, providing detailed, readily reproducible
methods, tips, and alternatives directly and easily transferable to
the laboratory setting.
Proteins are the functional units of the cellular machinery and
they provide significant information regarding the molecular basis
of health and disease. Therefore, techniques to separate and
isolate the various proteins are critical to studying and
understanding their functional characteristics. One of the widely
used techniques for this purpose is electrophoresis. In Protein
Electrophoresis: Methods and Protocols, contributions from experts
in the field have been collected in order to provide practical
guidelines to this complex study. Each chapter outlines a specific
electrophoretic variant in detail so that laboratory scientists may
perform a technique new to their lab without difficulty. Written in
the successful Methods in Molecular Biology (TM) series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible protocols, and notes on troubleshooting and avoiding
known pitfalls. Authoritative and accessible, Protein
Electrophoresis: Methods and Protocols seeks to serve laboratory
scientists with well-honed, detailed methodologies in an effort to
further our knowledge of this essential field.
This book fills the need for a simplified text covering western
blotting protocols aimed not just at high school and college
students, but the researcher with little to no experience in these
techniques. It provides the principles, basic methodology, and tips
and tricks to avoiding the common pitfalls of western blotting. The
book also introduces simple protocols that can transform western
blotting into a fun method, such as sending secret messages on
membranes or using nitrocellulose membrane as a canvas for art. In
addition to the techniques, this book also covers the history of
western blotting, which originated from the development of the
blotting of DNA. It then delves into the importance of protein
blotting, brought to the fore by the fact that the procedure has
been evolving constantly since its inception in 1979, and the fact
that the scientific community is faced with a multitude of ways and
means of transferring proteins to membranes..
Proteins are the functional units of the cellular machinery and
they provide significant information regarding the molecular basis
of health and disease. Therefore, techniques to separate and
isolate the various proteins are critical to studying and
understanding their functional characteristics. One of the widely
used techniques for this purpose is electrophoresis. In Protein
Electrophoresis: Methods and Protocols, contributions from experts
in the field have been collected in order to provide practical
guidelines to this complex study. Each chapter outlines a specific
electrophoretic variant in detail so that laboratory scientists may
perform a technique new to their lab without difficulty. Written in
the successful Methods in Molecular Biology (TM) series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible protocols, and notes on troubleshooting and avoiding
known pitfalls. Authoritative and accessible, Protein
Electrophoresis: Methods and Protocols seeks to serve laboratory
scientists with well-honed, detailed methodologies in an effort to
further our knowledge of this essential field.
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