Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results.
Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations.
Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician.
- Provides an overview of platforms
- Includes experimental design and wet bench protocols
- Presents statistical and data analysis methods, array databases, data visualization and meta analysis

Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements from a single experiment. These two volumes, Parts A & B in the Methods in Enzymology series provide methods that will shepard any molecular biologist through the process of planning, performing, and publishing microarray results.
Part A starts with an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no difficulty generating beautiful array hybridizations.
Data management is a more recent problem for most biologists. The bulk of Part B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B for both the debutant and the expert bioinformatician.
- Provides an overview of platforms
- Includes experimental design and wet bench protocols
- Presents statistical and data analysis methods, array databases, data visualization and meta analysis

Psychological thriller set in the American ballet world starring Natalie Portman in an award-winning performance. Nina (Portman) is a dancer in a New York City ballet company. Still living with her domineering and obsessive mother Erica (Barbara Hershey), a former ballerina herself, Nina barely has a life outside her dancing. When the company's artistic director Thomas Leroy (Vincent Cassel) decides to replace prima ballerina Beth MacIntyre (Winona Ryder) for a major new production of Swan Lake, Nina finds herself losing sight of her own identity as she becomes caught up in a twisted competitive friendship with her rival Lily (Mila Kunis). Portman won the 2011 Golden Globe, BAFTA and Academy Award for Best Actress.

Follow Newie's next journey of adventure and courage to leave his small town to pursue his dream in the big city of New York.

Learn the simple secrets to making your paintings work. Included are full of explanations and images to help create beautiful outdoor paintings. Demonstrations emphasize the important points learned in each chapter and show how to achieve desired results. Let Brian Oliver show you how to take your paintings to the next level

HOW (NOT) TO WRITE A HIT SONG - 101 COMMON MISTAKES TO AVOID IF YOU WANT SONGWRITING SUCCESS takes a close look at the essential elements consistently found in the structure, melodies and lyrics of all hit songs. The book highlights and analyzes the most common errors that are made when these key components are built into a song-so that new writers can try to avoid such mistakes in their own songs. Most writers have, at some stage, had to endure the disappointment of having songs rejected by music publishers or record companies-and ended up asking themselves: "Could I have done more to make my songs better?." This book aims to help writers recognise any weaknesses in their songs, so that they can re-work them, make them stronger, and hopefully achieve the breakthrough that they're seeking. The music industry has been going through major changes in recent years. It is now a 'singles market' again. As a result, there are now even greater opportunities for new writers who are capable of crafting potential hit songs with memorable melodies and lyrics. But competition is intense. So it's more important than ever for writers to make sure their songs stand out from the pack by avoiding the traps and pitfalls that many inexperienced songwriters fall into. Written in an easy, non-technical style, the book identifies many frequent causes of songwriting problems-from common mistakes in basic song structure ... to having the wrong mental attitude and an unsatisfactory songwriting environment. From getting the blend of core ingredients wrong ... to flawed choices when it comes to titles, melodies, lyrics, hooks, choruses, intros, bridges, pre-choruses-and even the song demo itself. The book's author, Brian Oliver-an experienced music publisher, songwriter and music consultant-warns that aspiring writers' chances of success could be hampered if they fail to spend enough time polishing their songs and eradicating weaknesses in their songwriting. That's why this book sets out to help new writers develop their own unique writing style while avoiding fundamental errors at each key stage in the song development process. It includes important tips on fixing and strengthening songs, along with a detailed checklist of 101 common mistakes that writers can measure their own songs against-no matter how 'finished' they think their songs are.

Brian Helgeland writes and directs this crime thriller starring Tom Hardy in the dual role of infamous gangster twins Ronald and Reginald Kray. The film chronicles the Kray twins' career during the 1950s and '60s as they tighten their tyrannical rule over London. With Ronnie's mental stability in question Reggie attempts to keep him under control while embarking on a romance with the beautiful Frances Shea (Emily Browning). Meanwhile, Detective Superintendent Leonard 'Nipper' Read (Christopher Eccleston) tries to bring the Krays down. The cast also includes David Thewlis, Colin Morgan, Taron Egerton and Tara Fitzgerald.

Brian Helgeland writes and directs this crime thriller starring Tom Hardy in the dual role of infamous gangster twins Ronald and Reginald Kray. The film chronicles the Kray twins' career during the 1950s and '60s as they tighten their tyrannical rule over London. With Ronnie's mental stability in question Reggie attempts to keep him under control while embarking on a romance with the beautiful Frances Shea (Emily Browning). Meanwhile, Detective Superintendent Leonard 'Nipper' Read (Christopher Eccleston) tries to bring the Krays down. The cast also includes David Thewlis, Colin Morgan, Taron Egerton and Tara Fitzgerald.