The molecular basis of disease is the driving force in biomedical reserach. Gene detection, quantification, and localization are required to determine disease pathogenesis. In as much as DNA is the genetic potential in a particular organism, RNA and protein expression are the functional result of our genetic program. "Techniques in Localization of Gene Expression" describes in great detail the methods used to quantify and localize gene expression. Sensitive detection of gene expression is hindered by poor recovery or preservation of target sequences. "Techniques" emphasizes optimized methods to ensure full recovery and preservaiton of targets from specimen acquisition through detection of specific targets and final analysis. Situ Analyses of cells and tissues are also discussed. One of the weaknesses in biomedical research has been the use of in vitro model systems consisting of cell lines that may or may not mimic the in vivo conditions. These model systems are necessary to ask several questions on cell s and tissues with controllled variables int he absense of strategies to ask many questions at the same time. Multiparameter analysis can cirmcumvent the necessity fo in vitro model systems by allowing many quesitons to be answered on a cell or tissue at a single point in time. Using fluorescent dyes, on can answer many questions about a particular cell or tissue. One color, one question is an overriding theme of this book. The limitations of this approach are the number of colors in the spectrum and our ability to resolve them. Readers will find working protocols that have been developed and used routinely in the author's laboratory. Representative applications will be described using the various gene quantification and localization protocols to illustrate the utility of the techniques.

The molecular basis of disease is the driving force in biomedical reserach. Gene detection, quantification, and localization are required to determine disease pathogenesis. In as much as DNA is the genetic potential in a particular organism, RNA and protein expression are the functional result of our genetic program. Techniques in Localization of Gene Expression describes in great detail the methods used to quantify and localize gene expression. Sensitive detection of gene expression is hindered by poor recovery or preservation of target sequences. Techniques emphasizes optimized methods to ensure full recovery and preservaiton of targets from specimen acquisition through detection of specific targets and final analysis. Situ Analyses of cells and tissues are also discussed. One of the weaknesses in biomedical research has been the use of in vitro model systems consisting of cell lines that may or may not mimic the in vivo conditions. These model systems are necessary to ask several questions on cell s and tissues with controllled variables int he absense of strategies to ask many questions at the same time.Multiparameter analysis can cirmcumvent the necessity fo in vitro model systems by allowing many quesitons to be answered on a cell or tissue at a single point in time. Using fluorescent dyes, on can answer many questions about a particular cell or tissue. One color, one question is an overriding theme of this book. The limitations of this approach are the number of colors in the spectrum and our ability to resolve them. Readers will find working protocols that have been developed and used routinely in the author's laboratory. Representative applications will be described using the various gene quantification and localization protocols to illustrate the utility of the techniques.

REALITY CAN ONLY BE REAL, IF IT IS TRUE.
Finally, Science, Evolution, Creationism and Intelligent Design combine to form a coherent, comprehensive and logical reality without going into Religion or Politics. This book proves the theory of the "Big Bang" and its ramifications that supposedly led to the existence of the human race and the universe are not real and could never be real. Regardless of which line of thought you have an affinity for, you will be amazed at how they seem to fit together to form a mosaic of reality that the scientist cannot deny, evolutionists cannot invent, the creationists can accept and the intelligent designers' will revel in. This book demonstrates that Truth is stranger and more fantastic than fiction.