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The papers assembled in this collection comprise a majority of the
oral presentations as well as several poster presentations given at
the 22nd Annual Symposium arranged by the Bastern Pennsylvania
Branch of the American Society for MicrobioloS)'. The symposium
would not be possible without the generous support of the many
sponsors (see sponsor list) or without the concerted effort of a11
the Committee members. This Symposium series has evolved into an
annual Bastern Pennsylvania Branch ASM event that attracts
participants from a wide geographie area. It should be noted that
one of the hallmarks of these symposia involves interaction between
the presenters and those in attendance. Several authors have
altered their by the participants. Therefore, the manuscript that
manuscripts based on comments fo11ows should be viewed as a group
effort of both the participants and presenters. J ames Poupard Lori
Walsh Bruee Kleger ix CONTENTS 1 Introduction 1: CURRENT METHODS
The Evolution of Antimicrobial Susceptibility Testing Methods . . .
. . . . . . . . . . . . 3 James A. Poupard, Stephen F. Rittenhouse,
and Lori R. Walsh Antimicrobial Susceptibility Tests: Testing
Methods and Interpretive Problems. . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . 15 Patrick R. Murray Clinician
Utilization of Rapid Antibiotic Susceptibility Data: A Prospective
Study . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . 27 Franklin P. Koontz SESSION 2: CONTEMPORARY ISSUES IN
SUSCEPTIBILITY TESTING When We Should Be Testing, How Often and
What to Report . . . . . . . . . . . . . . . 35 Raymond C. Bartlett
Areas of Recent Emphasis of the National Committee for Clinical
Laboratory Standards Subcommittee on Antimicrobial Susceptibility
Testing . . . . . . . . . . . . . . . . . . . . . . . 61 James H.
Jorgensen Non-Traditional Approaches for Quality Control of
Antimicrobial Susceptibility Tests . . . . . . . . . . . . . . . .
. . . . . . . . .
When we were setting the theme of "infection control dilemmas and
practical solutions" for this symposium, we asked ourselves a basic
question: What are some of the most vexing problems and situations
facing the hospital microbiologist epidemiologist team in today's
world of opportunistic and new infectious diseases unheard of as
common pathogenic occurrences 10 years ago? One of the areas which
we immediately focused upon was the tremendous amount of time,
energy, and financial resources that are presently being expended
to satisfy the requirements mandated by the recognition of the
danger of spread of blood-borne pathogens in the hospital
environment. With the advent of Universal Precautions, primarily in
response to HIV infection and the AIDS crisis, but certainly
augmented by the increased incidence of hepatitis in its various
forms, a significant effort has been required to meet the standards
rec ommended and/or required by OSHA and the CDC. With this in mind
we brought together experts in the field of infectious diseases to
address the problems engendered by the threat of nosocomial spread
of selected pathogens. Further, we devoted several sessions to
discussing the investi gation and resolution of institutional
outbreaks of disease, particularly with reference to
methicillin-resistant Staphylo coccus aureus (MRSA). Special
problems of dental offices and clinical teaching as well as
extended care facilities were also selected for attention,
particularly with relation to blood borne pathogens."
The papers published herein comprise the presentations given at the
eighteenth of an annual series of clinical symposia arranged under
the auspices of the Eastern Pennsylvania Branch of the American
Society for Microbiology. This symposium allowed approximately 200
persons to gather and exchange ideas on the rapid laboratory
diagnosis of infectious diseases. The institution of the Diagnosis
Related Group (DRG) method for reimbursement by both government
agencies and private insurance carriers has provided a financial
aspect to the established clinical reasons for rapid laboratory
diagnosis. Now the health of the institution, as well as the
patient, is dependent on a timely diagnosis and, hopefully, cure.
Accordingly, the goal of this symposium was to present the latest
developments in "same-day microbiology." In the face of stable or
diminishing resources, the laboratory director is presented with
many choices. Do nucleic acid probes, non instrumental ELISA
techniques, or time-resolved fluorometry have a place in his or her
laboratory? Should the laboratory test for newly described human
pathogens such as human immunodeficiency virus or human papilloma
virus? Can rapid techniques supplant conventional methods? Or are
they merely adjunctive? This symposium attempted to assist in the
formulation of informed decisions. Bruce Kleger Donald Jungkind
Eileen Rinks Linda A. Miller vii ACKNOWLEDGEMENTS We would like to
thank the Eastern Pennsylvania Branch of t e American Society for
Microbiology for sponsoring this symposium and for making this
publication possible. We especially thank the Symposium Committee
for their diligent work in organizing an informative and successful
symposium."
The papers assembled in this collection comprise a majority of the
oral presentations as well as several poster presentations given at
the 22nd Annual Symposium arranged by the Bastern Pennsylvania
Branch of the American Society for MicrobioloS)'. The symposium
would not be possible without the generous support of the many
sponsors (see sponsor list) or without the concerted effort of a11
the Committee members. This Symposium series has evolved into an
annual Bastern Pennsylvania Branch ASM event that attracts
participants from a wide geographie area. It should be noted that
one of the hallmarks of these symposia involves interaction between
the presenters and those in attendance. Several authors have
altered their by the participants. Therefore, the manuscript that
manuscripts based on comments fo11ows should be viewed as a group
effort of both the participants and presenters. J ames Poupard Lori
Walsh Bruee Kleger ix CONTENTS 1 Introduction 1: CURRENT METHODS
The Evolution of Antimicrobial Susceptibility Testing Methods . . .
. . . . . . . . . . . . 3 James A. Poupard, Stephen F. Rittenhouse,
and Lori R. Walsh Antimicrobial Susceptibility Tests: Testing
Methods and Interpretive Problems. . . . . . . . . . . . . . . . .
. . . . . . . . . . . . . . 15 Patrick R. Murray Clinician
Utilization of Rapid Antibiotic Susceptibility Data: A Prospective
Study . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
. . . 27 Franklin P. Koontz SESSION 2: CONTEMPORARY ISSUES IN
SUSCEPTIBILITY TESTING When We Should Be Testing, How Often and
What to Report . . . . . . . . . . . . . . . 35 Raymond C. Bartlett
Areas of Recent Emphasis of the National Committee for Clinical
Laboratory Standards Subcommittee on Antimicrobial Susceptibility
Testing . . . . . . . . . . . . . . . . . . . . . . . 61 James H.
Jorgensen Non-Traditional Approaches for Quality Control of
Antimicrobial Susceptibility Tests . . . . . . . . . . . . . . . .
. . . . . . . . .
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