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This new volume of "Methods in Enzymology" continues the legacy of
this premier serial by containing quality chapters authored by
leaders in the field. The volume covers nucleosomes, histones and
chromatin and has chapters on dynamic mapping of histone-DNA
interactions in nucleosomes by unzipping single molecules of DNA,
digital DNase technology, and genome-wide analysis of chromatin
transition.
This new volume of "Methods in Enzymology" continues the legacy of
this premier serial by containing quality chapters authored by
leaders in the field. The first of 2 volumes covering nucleosomes,
histones and chromatin, it has chapters on methods applied to the
study of protein arginine methylation, high-resolution
identification of intra- and interchromosomal DNA interactions by
4C technology, and peptide arrays to interrogate the binding
specificity of chromatin-binding proteins.
DNA in the nucleus of plant and animal cells is stored in the form
of chromatin. Chromatin and the chromatin remodelling enzymes play
an important role in gene transcription.
DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodelling enzymes play an important role in gene transcription.
The regulation of gene expression in many biological processes involves epigenetic mechanisms. In this new volume, 36 chapters written by experts in the field discuss epigenetic effects from many perspectives. There are chapters on the basic molecular mechanisms underpinning epigenetic regulation, discussion of cellular processes that rely on this kind of regulation, and surveys of organisms in which it has been most studied. Thus, there are chapters on histone and DNA methylation, siRNAs and gene silencing; X-chromosome inactivation, dosage compensation and imprinting; and discussion of epigenetics in microbes, plants, insects, and mammals. The last part of the book looks at how epigenetic mechanisms act in cell division and differentiation, and how errors in these pathways contribute to cancer and other human diseases. Also discussed are consequences of epigenetics in attempts to clone animals. This book is a major resource for those working in the field, as well as being a suitable text for advanced undergraduate and graduate courses on gene regulation.
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