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New insights into modern medicine and systems biology are enabled
by innovative protocols and advanced technologies in mass
spectrometry-based proteomics. This volume details new pipelines,
workflows, and ways to process data that allow for new frontiers in
proteomics to be pushed forward. With applications to biomarker
discovery, interactions between proteins, between biological
systems, dynamics of post-translational modifications among others,
new protocols have been developed and iteratively refined to probe
the endless complexity of the proteome in ever greater details.
This volume deals with methods for data dependent and data
independent mass spectrometry analyses. Valuable, first-hand
information is provided from designing experiments, sample
preparation and analysis, exploitation of public datasets and
carrying out reproducible data pipelines, using modern
computational tools such as Galaxy or Jupyter. Written in the
highly successful Methods in Molecular Biology series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and cutting-edge, Mass
Spectrometry of Proteins: Methods and Protocols aims to ensure
successful results in the further study of this vital field.
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