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Amino acid analysis is a technique that has become commonplace in biotechnology, biomedical, and food analysis laboratories. This book describes a variety of amino acid analysis techniques and how each technique can be used to answer specific biological questions. The first two chapters in Amino Acid Analysis Protocols introduce the concepts, basic theory, and practice of amino acid analysis. The following chapters give detailed instructions on various methods and their applications. As highlighted, there are many different approaches to amino acid ana- sis, but in all cases the results depend heavily on the quality of the sample. Therefore a new way to desalt samples prior to hydrolysis is covered as an introductory chapter (Chapter 3), and most authors have devoted a section to sample preparation, especially to the collection and storage of bodily fluids. Some of the amino acid analysis methods described in this book are based on HPLC separation and analysis after precolumn derivatization. The precolumn derivatization techniques described use (a) 6-aminoquinolyl-N-hydro- succinimidyl carbamate (AQC) (Chapters 4 and 8); (b) 1-fluoro-2- dinitrophenyl-5-L-alanine amide (Marfey's reagent), which allows sepa- tion and analysis of enantiomeric amino acids (Chapter 5); (c) O-phthalal- hyde (OPA) (Chapters 6 and 10); (d) butylisothiocyanate (BITC) and benzylisothiocyante (BZITC) (Chapter 11); (e) phenylisothiocyanate (PITC) (Chapters 12 and 13); (f) ammonium-7-fluorobenzo-2-oxa-1,3-diazole-4-s- fonate (SBD-F) (Chapter 17); and (g) 9-fluorenylmethyl-chloroformate (FMOC-Cl) (Chapter 10).

**The Sunday Times Top 5 bestseller** Longlisted for the CWA New Blood Award Four friends. One luxury getaway. The perfect murder. ‘Pure adrenaline’ ERIN KELLY ‘An intense thriller’ HEAT ‘Agatha Christie meets the glamour of après-ski’ SUNDAY TIMES French Alps, 1998 Two young men ski into a blizzard… but only one returns. 20 years later Four people connected to the missing man find themselves in that same resort. Each has a secret. Two may have blood on their hands. One is a killer-in-waiting. Someone knows what really happened that day. And somebody will pay. An exciting new debut for anyone who loves Ruth Ware, Lucy Foley, and C.L. Taylor ‘An intense, claustrophobic thriller’ Heat magazine ‘A brilliant book with a twist you won’t see coming’ Bella magazine ‘A great, pacy read fans of Lucy Foley will love’ Fabulous magaazine ‘Atmospheric and suspenseful’ Woman’s Weekly ‘A fast-paced and easy-to-read thriller, with a gorgeously escapist setting’ Best magazine ‘I was gripped from start to finish’ Cass Green ‘Chilling and atmospheric’ Roz Watkins ‘Thrilling – I could feel the icy chill blowing through the pages’ Michelle Frances ‘Cleverly plotted’ Allie Reynolds ‘The perfect book to take a stay-cation with!’ Suzy K Quinn

The gripping new thriller from the Sunday Times bestseller! ‘Brilliantly escapist in every way’ Editor’s Choice, The Bookseller Praise for Catherine Cooper: ‘A brilliant new storyteller has arrived’ ERIN KELLY ‘Intense and claustrophobic’ HEAT ‘Agatha Christie with glamour’ SUNDAY TIMES The perfect escape, or the perfect trap? When a select group of influencers and journalists receive an exclusive invitation to a luxury resort in the Maldives, it seems like the ultimate press trip. But when the island is cut off during a storm and people start dying, it looks like someone has murder in mind. Are the guests really who they seem to be, or does each one of them have a secret to hide? Something they would kill for? Lose yourself in the latest twisty page-turner from the queen of glamorous crime, Catherine Cooper.

A glamorous ship. A missing woman. A holiday to DIE for… The gripping new thriller from the Sunday Times bestseller! ‘A brilliant new storyteller has arrived’ ERIN KELLY ‘Intense and claustrophobic’ HEAT ‘Agatha Christie with glamour’ SUNDAY TIMES A glamorous ship During a New Year’s Eve party on a large, luxurious cruise ship in the Caribbean, the ship’s dancer, Lola, goes missing. Everyone on board has something to hide Two weeks later, the ship is out of service, laid up far from land with no more than a skeleton crew on board. And then more people start disappearing… No one is safe Why are the crew being harmed? Who is responsible? And who will be next? Find out in the twisty new thriller from the queen of glamorous crime, Catherine Cooper. ‘A well-plotted twisty read from the destination thriller author’ WOMAN’S WEEKLY PRAISE FOR CATHERINE COOPER: ‘A brilliant book with a twist you won’t see coming’ BELLA ‘A great, pacy read fans of Lucy Foley will love’ FABULOUS ‘Atmospheric and suspenseful’ WOMAN’S WEEKLY ‘A striking debut – the ultimate winter read!’ FRANCE MAGAZINE ‘Adrenaline-charged – makes for superb, wintry reading’ WI LIFE ‘I was gripped by The Chalet from start to finish and loved the vivid setting’ CASS GREEN ‘I LOVED this fun, fast-paced murder mystery. Luxurious surroundings, great characters and dark secrets’ SUZY K QUINN ‘A chilling and atmospheric thriller full of dark secrets and addictive twists' ROZ WATKINS ‘A cleverly plotted thriller set almost entirely in the French Alps. The descriptions of the snow made me feel like I was there’ ALLIE REYNOLDS ‘A striking debut… the ultimate winter read’ France magazine ‘The plot is as chilling as the setting: this is the perfect read for a cold winter's evening ’ ROBIN MORGAN-BENTLEY ‘An absolutely cracking read’ NELL PATTISON ‘Great suspense with more twists and turns than off-piste skiing’ LAURE VAN RENSBURG ‘A fantastic setting for a chilling thriller’ CHRIS MCDONALD ‘A perfect atmospheric whodunnit’ DIANE JEFFERY ‘A perfect winter read’ DEBBIE HOWELLS

Amino acid analysis is a technique that has become commonplace in biotechnology, biomedical, and food analysis laboratories. This book describes a variety of amino acid analysis techniques and how each technique can be used to answer specific biological questions. The first two chapters in Amino Acid Analysis Protocols introduce the concepts, basic theory, and practice of amino acid analysis. The following chapters give detailed instructions on various methods and their applications. As highlighted, there are many different approaches to amino acid ana- sis, but in all cases the results depend heavily on the quality of the sample. Therefore a new way to desalt samples prior to hydrolysis is covered as an introductory chapter (Chapter 3), and most authors have devoted a section to sample preparation, especially to the collection and storage of bodily fluids. Some of the amino acid analysis methods described in this book are based on HPLC separation and analysis after precolumn derivatization. The precolumn derivatization techniques described use (a) 6-aminoquinolyl-N-hydro- succinimidyl carbamate (AQC) (Chapters 4 and 8); (b) 1-fluoro-2- dinitrophenyl-5-L-alanine amide (Marfey's reagent), which allows sepa- tion and analysis of enantiomeric amino acids (Chapter 5); (c) O-phthalal- hyde (OPA) (Chapters 6 and 10); (d) butylisothiocyanate (BITC) and benzylisothiocyante (BZITC) (Chapter 11); (e) phenylisothiocyanate (PITC) (Chapters 12 and 13); (f) ammonium-7-fluorobenzo-2-oxa-1,3-diazole-4-s- fonate (SBD-F) (Chapter 17); and (g) 9-fluorenylmethyl-chloroformate (FMOC-Cl) (Chapter 10).

THE TOP TWENTY SUNDAY TIMES BESTSELLER 'A brilliant new story-teller has arrived' ERIN KELLY 'A read-in-one-sitting thriller' LUCY CLARKE 'Chilling, devious' JANICE HALLETT 'Glorious escapism with a murderous twist' TAMMY COHEN They thought it was perfect. They were wrong... A glamorous chateau Aura and Nick don't talk about what happened in England. They've bought a chateau in France to make a fresh start, and their kids need them to stay together - whatever it costs. A couple on the brink The expat community is welcoming, but when a neighbour is murdered at a lavish party, Aura and Nick don't know who to trust. A secret that is bound to come out... Someone knows exactly why they really came to the chateau. And someone is going to give them what they deserve. The Sunday Times bestseller is back with a rollercoaster read, perfect for fans of Lucy Foley and Ruth Ware. 'Gripping, glamorous, ingeniously twisty' LAURA MARSHALL 'The definition of unputdownable' SINEAD CROWLEY 'Hot on the heels of her debut thriller, Cooper has crafted yet another gripping page-turner that kept me second guessing myself until the very end' KARIN NORDIN Praise for Catherine Cooper: 'Pure adrenaline' Erin Kelly 'Agatha Christie with glamour' Sunday Times Style 'Intense and claustrophobic' Heat The Chalet was a Sunday Times No.5 bestseller for w/e 28/11/20