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Protein microarrays have been used for a wide variety of important
tasks, such as identifying protein-protein interactions,
discovering disease biomarkers, identifying DNA-binding specificity
by protein variants, and for characterization of the humoral immune
response. In Protein Microarray for Disease Analysis: Methods and
Protocols, expert researchers provide concise descriptions of the
methodologies currently used to fabricate microarrays for the
comprehensive analysis of proteins or responses to proteins that
can be used to dissect human disease. These methodologies are the
toolbox for revolutionizing drug development and cell-level
biochemical understanding of human disease processes. Beginning
with a section on protein-detecting analytical microarrays, the
volume continues with sections covering antigen microarrays for
immunoprofiling, protein function microarrays, the validation of
candidate targets, proteomic libraries, as well as signal detection
strategies and data analysis techniques. Written in the highly
successful Methods in Molecular Biology (TM) series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and key tips on troubleshooting
and avoiding known pitfalls. Practical and cutting-edge, Protein
Microarray for Disease Analysis: Methods and Protocols serves as a
solid framework to aid scientists in understanding how protein
microarray technology is presently developing and how it can be
applied to transform our analysis of human disease.
Protein microarrays have been used for a wide variety of important
tasks, such as identifying protein-protein interactions,
discovering disease biomarkers, identifying DNA-binding specificity
by protein variants, and for characterization of the humoral immune
response. In Protein Microarray for Disease Analysis: Methods and
Protocols, expert researchers provide concise descriptions of the
methodologies currently used to fabricate microarrays for the
comprehensive analysis of proteins or responses to proteins that
can be used to dissect human disease. These methodologies are the
toolbox for revolutionizing drug development and cell-level
biochemical understanding of human disease processes. Beginning
with a section on protein-detecting analytical microarrays, the
volume continues with sections covering antigen microarrays for
immunoprofiling, protein function microarrays, the validation of
candidate targets, proteomic libraries, as well as signal detection
strategies and data analysis techniques. Written in the highly
successful Methods in Molecular Biology (TM) series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and key tips on troubleshooting
and avoiding known pitfalls. Practical and cutting-edge, Protein
Microarray for Disease Analysis: Methods and Protocols serves as a
solid framework to aid scientists in understanding how protein
microarray technology is presently developing and how it can be
applied to transform our analysis of human disease.
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