UV-VIS spectroscopy was discovered long ago and Beckman brought the first spectrophotometer. The analysis of the overlapping bands in electronic spectra is difficult. In such circumstances derivative spectroscopy becomes a very simple and powerful tool of analysis. The use of differentiation to enhance the fine structure of empirical data was first proposed by Lord Rutherford in the early 1920s. The chapters in this books are as: Chapter 1 describes basics and fundamentals of the phenomenon involved. Chapter 2 reviews the related and relevant work and at the end of this chapter we have defined the objectives of the project. Chapter 3 describes the methodology and protocol adapted to complete this study. Chapter 4 describes the results. The discussions have been provided alongside and conclusions have been added at the end of each subsectio. Chapter 5 summarises the overall conclusions of the whole project. As it is said that no study is a closed end project, but it lays the some foundation on which future observations are added up.