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This volume looks the current advanced protocols used to study
aspects of the B cell receptor (BCR). The chapters in this book
cover topics such as the mutant of BCR repertoire to understand
antibody evolution; interactions between B cells and viruses;
mechanical force during BCR activation; B cell signaling using flow
cytometry; confocal microscopy, total internal reflection
microscopy and intravital two-photon microscopy; and the methods
used to study critical cell components related with B cell
activation. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
practical, B Cell Receptor Signaling: Methods and Protocols is a
valuable resource for everyone in the scientific community
currently working in the B cell field.
This detailed book focuses on various aspects of Invariant Natural
Killer T-Cells (iNKT), which are known to contribute to homeostasis
and autoimmunity and can also cause various pathological responses
such as allergy, infection, excessive autoimmune response, and
cancer. The volume explores techniques for identification and
isolation of iNKT cells, iNKT cell activation and transformation,
as well as proliferation and differentiation and much more. Written
for the highly successful Methods in Molecular Biology series,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Invariant
Natural Killer T-Cells: Methods and Protocols is an ideal guide for
researchers working with and studying iNKT cells, perhaps to
provide a deeper knowledge of the human immune system.
This detailed book focuses on various aspects of Invariant Natural
Killer T-Cells (iNKT), which are known to contribute to homeostasis
and autoimmunity and can also cause various pathological responses
such as allergy, infection, excessive autoimmune response, and
cancer. The volume explores techniques for identification and
isolation of iNKT cells, iNKT cell activation and transformation,
as well as proliferation and differentiation and much more. Written
for the highly successful Methods in Molecular Biology series,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Invariant
Natural Killer T-Cells: Methods and Protocols is an ideal guide for
researchers working with and studying iNKT cells, perhaps to
provide a deeper knowledge of the human immune system.
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