Biochemical Bottom Lines presents the basic concepts covered in one-semester biochemistry courses, without all of the extraneous details found in most texts. It is a concise and manageable presentation of the basic biochemical principles together with problem-solving and review tools that keeps students focused on the essential concepts they need to learn. In the one-semester biochemistry course, most students feel overwhelmed by the amount of material covered in lectures and the task of identifying exactly what they should take with them upon completion of the course; therefore, Hardin's Biochemical Bottom Lines extracts and presents the essential core concepts imbedded in a typical biochemistry textbook and explains them in a variety of contexts, so students are more likely to gain a conceptual understanding of the processes of biochemistry. Biochemical Bottom Lines serves as an excellent background for those students that go on to take more advanced or more in-depth biochemistry courses. To further reinforce core concepts, the last 40% of the book is devoted to a set of review questions and practice tests.The text is divided into 30 chapters and designed to be assigned as supplement to the main text in the course, however it can be used as a stand alone text.

This combined lecture and laboratory manual presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. The manual includes 20 experiments designed to train students to prepare, manipulate and analyse plasmids, to produce fusion proteins in bacteria, and to purify these proteins based on unique chemical properties or substrate affinities. Maximizing its utility for both students and instructors, this unique manual precedes each lab experiment with the necessary background theory and principles, which culminate in detailed process rationale followed by the actual experimental technique. While many lab manuals list background references, this innovative book combines both theory and procedure into one manageable volume.