Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Necrosis: Methods and Protocols describes techniques in an easy to follow manner, with details so that beginners can succeed with challenging techniques.

Purchase one of 1st World Library's Classic Books and help support our free internet library of downloadable eBooks. Visit us online at www.1stWorldLibrary.ORG - - There was unwonted bustle in the usually sleepy and dignified New York offices of the Southern and Transcontinental Railroad Company in lower Broadway. The supercilious, well-groomed clerks who, on ordinary days, are far too preoccupied with their own personal affairs to betray the slightest interest in anything not immediately concerning them, now condes-cended to bestir themselves and, gathered in little groups, conversed in subdued, eager tones. The slim, nervous fingers of half a dozen haughty stenographers, representing as many different types of business femininity, were busily rattling the keys of clicking typewriters, each of their owners intent on reducing with all possible despatch the mass of letters which lay piled up in front of her. Through the heavy plate-glass swinging doors, leading to the elevators and thence to the street, came and went an army of messengers and telegraph boys, noisy and insolent. Through the open windows the hoarse shouting of news-venders, the rushing of elevated trains, the clanging of street cars, with the occasional feverish dash of an ambulance - all these familiar noises of a great city had the far-away sound peculiar to top floors of the modern sky-scraper. The day was warm and sticky, as is not uncommon in early May, and the overcast sky and a distant rumbling of thunder promised rain before night.

Cell death is an essential process in development, and a major contributor to a wide range of human diseases. Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Necrosis: Methods and Protocols describes techniques in an easy to follow manner, with details so that beginners can succeed with challenging techniques.