This unique introduction to the growing field of microfluidics applied to genomics provides an overview of the latest technologies and emphasizes its potential in answering important biological questions. Written by a physicist and a biologist, it offers a more comprehensive view than the previous literature. The book starts with key ideas in molecular biology, developmental biology and microtechnology before going on to cover the specifics of single cell analysis and microfluidic devices for single cell molecular analysis. Review chapters discuss the state-of-the art and will prove invaluable to all those planning to develop microdevices for molecular analysis of single cells. Methods allowing complete analysis of gene expression in the single cell are stressed - as opposed the more commonly used techniques that allow analysis of only a few genes at a time. As pioneers in the field, the authors understand how critical it is for a physicist to understand the biological issues and questions related to single cell analysis, as well for biologists to understand what microfluidics is all about. Aimed predominantly at graduate students, this book will also be of significant interest to scientists working in or affiliated with this field.

In developed countries, the food superstore or hypermarket has become the dominant mode of food distribution; and their success has transformed the retail sector. But each new store has changed the employment regime of the stores they replace. For retailers, labour is a major cost; with opening hours being extended, and consumer demand patterns changing, one solution has been the use of part-time labour. Extensive use of such labour characterises British food retailing for example whereas it is less common in France. This book examines the reasons for such differences, and the potential which management and employees have within the operation to manage working time to their benefit. This book makes use of a four country research programme, covering France, Germany, Great Britain and Japan. Investigations and interviews at store, company and individual level, paint a picture of working time in the sector and in each of the countries. The volume provides some explanations for national differences as well as the similarities; supply and demand issues, as well as societal and social backgrounds. Large format food retailing is a major force in each country, employing millions in many di