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There have been significant advances in research involving the
isolation and culture of epithelial cells in the past decade, and
many new techniques have been developed. Monolayer cultures can be
used to evaluate the nature and behavior of cells, while the use of
epithelial cells in model systems has allowed a deeper
understanding of cellular and molecular mechanisms and
interactions. The aim of this book is to provide a comprehensive,
step-by-step guide to many techniques for epithelial cell culture,
combining in one volume the more commonly used protocols along with
many that are more speci- ized. Epithelial Cell Culture Protocols
should help those who are new to this field and want to learn the
basic culture techniques, as well as those needing to use more wide
ranging and specific protocols. It should be a useful resource on
its own, and also complement the other volumes that have been
written about cell culture in the Methods in Molecular Biology
series. Epithelial Cell Culture Protocols covers a wide variety of
protocols, mostly aimed at the researcher, but also a few aimed at
clinicians. The est- lishment and maintenance of primary cultures
derived from many different tissues and different species is
covered. Particular emphasis has been placed on protocols needed to
further analyze and assess epithelial cells, for example, by
looking at apoptosis and integrins and by measuring membrane capa-
tance and confluence. Using different co-culture techniques, it is
possible also to develop models to investigate many different
systems in vitro.
'A remarkable account of illness, loss and the power of sibling
love' The Times 'Wise's reflections on compassion fatigue are worth
the price of this book alone, but what you take away is something
splendid and unwearying: a sibling's devotion that feels remarkably
like what we mean when we talk of a stage of grace.' Telegraph
'Inspirational... profoundly uplifting' Daily Mail 'Heartbreaking
and inspiring in equal measure' Express 'This is a fantastic book
... Remarkable' Lorraine Kelly _______ A moving, thought-provoking
and surprisingly humorous book which is both a description of a
journey to death and a celebration of the act of living. Based on
Clare Wise's blog, which she started when she was first diagnosed
with cancer in 2013, Not That Kind of Love charts the highs and
lows of the last three years of Clare's life. The end result is not
a book that fills you with despair and anguish. On the contrary,
Not That Kind of Love should be read by everybody for its candour,
and for its warmth and spirit. Clare is an astonishingly dynamic,
witty and fun personality, and her positivity and energy exude from
every page. As she becomes too weak to type, her brother - the
actor Greg Wise - takes over, and the book morphs into a beautiful
meditation on life, and the necessity of talking about death. As
Greg Wise writes in the book: 'Celebrate the small things, the
small moments. If you find yourself with matching socks as you
leave the house in the morning, that is a cause for celebration. If
the rest of the day is spent finding the cure for cancer, or
brokering world peace, then that's a bonus.'
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