There have been significant advances in research involving the isolation and culture of epithelial cells in the past decade, and many new techniques have been developed. Monolayer cultures can be used to evaluate the nature and behavior of cells, while the use of epithelial cells in model systems has allowed a deeper understanding of cellular and molecular mechanisms and interactions. The aim of this book is to provide a comprehensive, step-by-step guide to many techniques for epithelial cell culture, combining in one volume the more commonly used protocols along with many that are more speci- ized. Epithelial Cell Culture Protocols should help those who are new to this field and want to learn the basic culture techniques, as well as those needing to use more wide ranging and specific protocols. It should be a useful resource on its own, and also complement the other volumes that have been written about cell culture in the Methods in Molecular Biology series. Epithelial Cell Culture Protocols covers a wide variety of protocols, mostly aimed at the researcher, but also a few aimed at clinicians. The est- lishment and maintenance of primary cultures derived from many different tissues and different species is covered. Particular emphasis has been placed on protocols needed to further analyze and assess epithelial cells, for example, by looking at apoptosis and integrins and by measuring membrane capa- tance and confluence. Using different co-culture techniques, it is possible also to develop models to investigate many different systems in vitro.

'A remarkable account of illness, loss and the power of sibling love' The Times 'Wise's reflections on compassion fatigue are worth the price of this book alone, but what you take away is something splendid and unwearying: a sibling's devotion that feels remarkably like what we mean when we talk of a stage of grace.' Telegraph 'Inspirational... profoundly uplifting' Daily Mail 'Heartbreaking and inspiring in equal measure' Express 'This is a fantastic book ... Remarkable' Lorraine Kelly _______ A moving, thought-provoking and surprisingly humorous book which is both a description of a journey to death and a celebration of the act of living. Based on Clare Wise's blog, which she started when she was first diagnosed with cancer in 2013, Not That Kind of Love charts the highs and lows of the last three years of Clare's life. The end result is not a book that fills you with despair and anguish. On the contrary, Not That Kind of Love should be read by everybody for its candour, and for its warmth and spirit. Clare is an astonishingly dynamic, witty and fun personality, and her positivity and energy exude from every page. As she becomes too weak to type, her brother - the actor Greg Wise - takes over, and the book morphs into a beautiful meditation on life, and the necessity of talking about death. As Greg Wise writes in the book: 'Celebrate the small things, the small moments. If you find yourself with matching socks as you leave the house in the morning, that is a cause for celebration. If the rest of the day is spent finding the cure for cancer, or brokering world peace, then that's a bonus.'