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"a gem of a textbook which manages to produce a genuinely fresh,
concise yet comprehensive guide" -Mark Leake, University of York
"destined to become a standard reference.... Not just a 'how to'
handbook but also an accessible primer in the essentials of kinetic
theory and practice." -Michael Geeves, University of Kent "covers
the entire spectrum of approaches, from the traditional steady
state methods to a thorough account of transient kinetics and rapid
reaction techniques, and then on to the new single molecule
techniques" -Stephen Halford, University of Bristol This
illustrated treatment explains the methods used for measuring how
much a reaction gets speeded up, as well as the framework for
solving problems such as ligand binding and macromolecular folding,
using the step-by-step approach of numerical integration. It is a
thoroughly modern text, reflecting the recent ability to observe
reactions at the single-molecule level, as well as advances in
microfluidics which have given rise to femtoscale studies. Kinetics
is more important now than ever, and this book is a vibrant and
approachable entry for anyone who wants to understand mechanism
using transient or single molecule kinetics without getting bogged
down in advanced mathematics. Clive R. Bagshaw is Emeritus
Professor at the University of Leicester, U.K., and Research
Associate at the University of California at Santa Cruz, U.S.A.
"a gem of a textbook which manages to produce a genuinely fresh,
concise yet comprehensive guide" -Mark Leake, University of York
"destined to become a standard reference.... Not just a 'how to'
handbook but also an accessible primer in the essentials of kinetic
theory and practice." -Michael Geeves, University of Kent "covers
the entire spectrum of approaches, from the traditional steady
state methods to a thorough account of transient kinetics and rapid
reaction techniques, and then on to the new single molecule
techniques" -Stephen Halford, University of Bristol This
illustrated treatment explains the methods used for measuring how
much a reaction gets speeded up, as well as the framework for
solving problems such as ligand binding and macromolecular folding,
using the step-by-step approach of numerical integration. It is a
thoroughly modern text, reflecting the recent ability to observe
reactions at the single-molecule level, as well as advances in
microfluidics which have given rise to femtoscale studies. Kinetics
is more important now than ever, and this book is a vibrant and
approachable entry for anyone who wants to understand mechanism
using transient or single molecule kinetics without getting bogged
down in advanced mathematics. Clive R. Bagshaw is Emeritus
Professor at the University of Leicester, U.K., and Research
Associate at the University of California at Santa Cruz, U.S.A.
L-------------------------------------------~ Kathy Ruppel, Ken
Niebling and JeffFiner for their help and comments on the first
draft. I am also grateful for discussions with and comments from Dr
Neil Miliar, Professor Bob Simmons, Dr Roger Cooke, Dr Tosbio
Yanagida, Dr John Kendrick-Jones, Dr Rob Cross, Dr Ian Trayer, Dr
John Sparrow, Dr Michael Geeves, Dr Bernhard Brennerand Dr Peter K.
night. The task of illustrating the book was made much easier by
the photographs and diagrams kindly provided by Professor Ken
Holmes, Dr Ron Milligan, Professor Basil Northover, Dr Hans
Warrick, Dr John Squire, Dr JetT Harford, Dr Mary Reedy, Dr A vril
Somlyo, Dr Darl Swartz, Dr Marion Greaser, Dr Peter Knight, Dr
Gerald OtTer, Dr Roger Craig, Dr Peter Vibert, Dr John
Kendrick-Jones, Dr Andrew Jackson, Dr Don Winkelmann, Dr Andrew
Sowerby and Dr Richard Ankrett. I am grateful to the Science and
Engineering Research Council for funding my travel to Stanford
University. Permission to reproduce copyrighted material from the
following publishers is gratefully acknowledged. The Physiological
Society (Figs 2. 6, 6. 1, 6. 11, 6. 12, 7. 6), The RockefeBer
University Press (Figs 3. 5, 4. 8, 8. 3), Academic Press(Figs3.
4,4. 4,4. 15, 9. 1, 9. 2), MacmillanPress(Figs 4. 2, 4. 3, 4. 12,
4. 13, 6. 6, 6. 9, 7. 8, 9. 3), Longman Group (Fig. 6. 7), The
Royal Society (Fig. 3. 7) and D. W. Fawcett (Fig. 3. 1).
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