This book constitutes the refereed proceedings of the Second International Conference on Applied Intelligence and Informatics, AII 2022, held in Reggio Calabria, Italy, during September 1-3, 2022. The 38 full papers included in this book were carefully reviewed and selected from 108 submissions. They were organized in topical sections as follows: Emerging Applications of AI and Informatics; Application of AI and Informatics in Healthcare; Application of AI and Informatics in Pattern Recognition; and Application of AI and Informatics in Network, Security, and Analytics.