This book constitutes the refereed proceedings of the 28th International Symposium on Graph Drawing and Network Visualization, GD 2020, which was held during September 16-18, 2020. The conference was planned to take place in Vancouver, Canada, but changed to an online format due to the COVID-19 pandemic. The 29 full and 9 short papers presented in this volume were carefully reviewed and selected from 82 submissions. They were organized in topical sections named: gradient descent and queue layouts; drawing tree-like graphs, visualization, and special drawings of elementary graphs; restricted drawings of special graph classes; orthogonality; topological constraints; crossings, k-planar graphs; planarity; graphs drawing contest.