This book provides a complete introduction to both the practical details and the theoretical foundations required in order to use electron microscopy as a research tool in structural biology. Planned and written by a group of 5 well-known experts who have pioneered different aspects of the field of electron cryo-microscopy (cryo-EM) of biological macromolecules, this book offers a depth of knowledge and expertise that could only be replicated from the primary literature with great difficulty. Figures and illustrations are used liberally to simplify the understanding of concepts and to provide ready access to examples of biological applications. In addition, numerous boxes provide auxiliary information for those who wish to go more deeply into areas of background with which they may not already be familiar. The organization of topics lends itself well to using this book either to gain a comprehensive mastery of all aspects of the field of cryo-EM or as an authoritative reference source for individual topics within the field.