This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammalian Synthetic Promoters: Methods and Protocols aims to aid researchers that are new to the field of synthetic promoters and inspire new developments.

The venue was the canteen block of the Red Hammer Cement Works. It was the usual set-up: way out of town, secretive directions to get there, and disco lights blazing... Moscow, 1993. The end of the Cold War and the collapse of the Soviet Union have brought unimaginable change to Russia. With this change come new freedoms: freedom to travel abroad and to befriend Westerners, freedom to make money, and even the freedom for an underground gay scene to take root. Encouraged by the new climate of openness, twenty-one-year-old Kostya ventures out of the closet and resolves to pursue his dreams: to work in the theatre and to find love as his idol Tchaikovsky never could. Those dreams, however, lead to tragedy - not only for Kostya, but for his mother and for the two young men he loves, as all three face up to the ways they have betrayed him. Last Dance at the Discotheque for Deviants is both a gripping mystery and a poignant, very human tale of people beset by forces beyond their control, in a world where all the old certainties have crumbled and it's far from clear what will eventually take their place.

Historians of rhetoric have long worked to recover women's education in reading and writing, but have only recently begun to explore women's speaking practices, from the parlor to the platform to the varied types of institutions where women learned elocutionary and oratorical skills in preparation for professional and public life. This book fills an important gap in the history of rhetoric and suggests new paths for the way histories may be told in the future, tracing the shifting arc of women's oratorical training as it develops from forms of eighteenth-century rhetoric into institutional and extrainstitutional settings at the end of the nineteenth century and diverges into several distinct streams of community-embodied theory and practice in the twentieth. Treating key rhetors, genres, settings, and movements from the early republic to the present, these essays collectively challenge and complicate many previous claims made about the stability and development of gendered public and private spheres, the decline of oratorical culture and the limits of women's oratorical forms such as elocution and parlor rhetorics, and women's responses to rhetorical constraints on their public speaking. Enriching our understanding of women's oratorical education and practice, this cutting-edge work makes an important contribution to scholarship in rhetoric and communication.

The Middle English poem Pearl is an elegy on the death of a child, written to provide consolation for the loss of a loved one through instruction in Christian theology. The mourning father's faith in God has been shaken by his daughter's death. Having fainted away on his daughter's grave, he is transported in a dream vision to an earthy paradise where he sees his daughter across a stream, transfigured and clothed in garments set with pearls. She explains to him her exalted status as a bride of Christ and shows him a vision of the New Jerusalem. Ravished with his vision, he attempts to cross the stream to join his Pearl, only to be woken from his dream of paradise. This translation of the poem is accompanied by a preface on the translation, a reproduction of the poem in its original orthography, a transcription of the manuscript, a glossary, and instructions on how to read the manuscript hand.

Today, no single issue dominates the global political landscape as much as terrorism. Aware of their unique position in the newly unipolar world, terrorist leaders - Osama bin Laden foremost among them - have articulated that economic warfare is a key component of the new terrorist agenda. Governments have accentuated the role of economic tools in their counter-terrorism policies while maintaining emphasis on the application of military force, or hard power, even though such tools often prove unnecessarily blunt, or in some cases are sorely inadequate. Given the complexity of the global threat posed by modern trans-national terrorist groups, combating terrorism with a mix of hard and soft power is more important than ever. The need for nuanced management and a full complement of choices in the policy toolkit is a pressing concern.Terrornomics is an invaluable new book for graduate and undergraduate courses in terrorism studies that - brings together contributions from renowned international scholars and practitioners from a variety of disciplines; provides a multifaceted view of contemporary financial counterterrorism and terrorist funding efforts; and, employs key concepts, terms, case studies and policy recommendations to advance the reader's understanding of the threats and possible courses of action. Terrornomics helps policy makers and students of the complex phenomenon known as terrorism grasp the critical financial and economic issues, while providing potential counterterrorist strategies.

This book is an invitation to explore some of the more puzzling aspects of the processes which are called `management'. It provides a focused collection of readings to support and encourage wider consideration of alternative ways of thinking about management, and its effectiveness, in contemporary organisations. Aimed particularly at the advanced management student, key features of this text include:
*a selection of contributions which derive from historically grounded and politically aware considerations, examining alternative approaches to understanding management.
*an exploration of the uncertainties and apparent contradictions encountered in management action.
*improved access to ideas which are fundamental to understanding management.

Contents:
Preface David Golding and David Currie 1. The Nature of Management David Golding 2. Encountering Management Vince Dispenza 3. Treading Treacle at Parties and Parapets - Excursions into Managerial Ideology Joe Nason 4. The Enactment of Political Tensions in Management - A Historical Perspective Judith Golding 5. Reconstructing The Study of Management Robert Protherough 6. Teaching Management Through Reflective Practice Janet McGivern and Jane Thompson 7. Circles of Uncertainty in Management Learning Judith Golding 8. Promises, Promotions and Pristine Porcelain - Rhetorics and Essences in Management Action Fred Dobson 9. Nothing Starts From Nowhere David Currie Reflective David Golding and David Currie.

Historians of rhetoric have long worked to recover women's education in reading and writing, but have only recently begun to explore women's speaking practices, from the parlor to the platform to the varied types of institutions where women learned elocutionary and oratorical skills in preparation for professional and public life. This book fills an important gap in the history of rhetoric and suggests new paths for the way histories may be told in the future, tracing the shifting arc of women's oratorical training as it develops from forms of eighteenth-century rhetoric into institutional and extrainstitutional settings at the end of the nineteenth century and diverges into several distinct streams of community-embodied theory and practice in the twentieth. Treating key rhetors, genres, settings, and movements from the early republic to the present, these essays collectively challenge and complicate many previous claims made about the stability and development of gendered public and private spheres, the decline of oratorical culture and the limits of women's oratorical forms such as elocution and parlor rhetorics, and women's responses to rhetorical constraints on their public speaking. Enriching our understanding of women's oratorical education and practice, this cutting-edge work makes an important contribution to scholarship in rhetoric and communication.

This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammalian Synthetic Promoters: Methods and Protocols aims to aid researchers that are new to the field of synthetic promoters and inspire new developments.

While basic techniques of test-driven development are simple to understand, real-world application requires knowledge of tools and techniques to effectively create, run and organize tests. This book bridges the gap between simple concepts and complex application. Ideal for you Java developers, this book explains how to use test-driven development to improve J2EE construction.

Not version-specific, this unprecedented book explains development tools and methodologies in conjunction with real-world cases and examples. It includes the use of open source unit testing frameworks such as JUnit and its extensions. The authors include complete stages: test coverage strategies, test organization, TDD incorporation, and automation. Two appendices are also included, for test planning and reference.

Electricity in its myriad forms has intrigued people for centuries. In our modern era it powers nearly every machine and modern convenience that defines contemporary life. However, by hiding power lines underground or in the walls of our homes and through generations of miniaturization, our sense of what electricity is and how we have come to understand it is not widely considered. Electricities is comprised of photographs that depict constructions Goldes refers to as 'performing still-lifes' based on historical experiments into the nature of electricity. Electrical phenomena including electrostatics, high-voltage arcing, Faraday's first transformer, water conductivity, electrified graphite drawings, and other inventions and experiments form the basis of these works. Elegant and playful, Goldes uses commonplace materials such as string, pins, wire, pencil lines and bright colored backgrounds in his ingenious investigations. The photographs reveal how electricity behaves; how it jumps gaps, repels, attracts, arcs, destroys, and often confounds our expectations. Uniting the strategies of art and science his visually rigorous images reveal a mechanistic understanding of electricity in dialogue with the viewer's subjectivities that can expand, build upon and even contradict such explanations.

CakePHP is a leading PHP-based web app development framework. When asking a question on forums or chat rooms, many CakePHP beginners get little help from the experts. Simple questions can get a response like, Well, just read the online manual and API. Unfortunately, the online manual is depreciated, and who wants to absorb a programming language or framework from an API? Beginning CakePHP will do the following: Leads you from a basic setup of CakePHP to building a couple applications that will highlight CakePHP's functionality and capabilities without delving too deeply into the PHP language, but rather what the CakePHP framework can offer the developer. Teaches you to use CakePHP by incorporating advanced features into your web development projects. Targets beginners of CakePHP or web frameworks in general as well as experienced developers with limited exposure to CakePHP. A secondary audience may include developers undecided on adopting CakePHP or business managers trying to assess the value of incorporating CakePHP into their toolbox. What you'll learn Install and configure the CakePHP web framework. Create your first CakePHP web application: to-do lists. Progress by building a more complex blogging application. Incorporate Ajax and Web 2.0 principles and techniques into your blogging and other CakePHP applications. Apply these sample applications along with advanced techniques as a framework or template for building your own web applications with CakePHP going forward. Who is this book for? This book will speak to an audience of developers already familiar with PHP but who may not be PHP experts. This book is tailored for those new to CakePHP and who want athorough tutorial. About the Apress Beginning Series The Beginning series from Apress is the right choice to get the information you need to land that crucial entry-level job. These books will teach you a standard and important technology from the ground up because they are explicitly designed to take you from novice to professional. You'll start your journey by seeing what you need to know--but without needless theory and filler. You'll build your skill set by learning how to put together real-world projects step by step. So whether your goal is your next career challenge or a new learning opportunity, the Beginning series from Apress will take you there--it is your trusted guide through unfamiliar territory Related Titles Beginning PHP and MySQL 5: From Novice to Professional, Second Edition

This new compilation of photographs of East Grinstead, its locality and people, comprises 180 unpublished images, mostly dating from c. 1902-3 onwards, and all but ten from the collection at East Grinstead Museum. Nearly all were taken by local professional photographers, and it is by individual photographer that the images are arranged in chronological order: William Page, Arthur Harding, Edgar Kinsey, Ernest Watts, Harold Connold and Malcolm Powell.

A compilation of key chapters from the top MK computer animation books available today - in the areas of motion capture, facial features, solid spaces, fluids, gases, biology, point-based graphics, and Maya. The chapters provide CG Animators with an excellent sampling of essential techniques that every 3D artist needs to create stunning and versatile images. Animators will be able to master myriad modeling, rendering, and texturing procedures with advice from MK's best and brightest authors.

Divided into five parts (Introduction to Computer Animation and Technical Background, Motion Capture Techniques, Animating Substances, Alternate Methods, and Animating with MEL for MAYA), each one focusing on specific substances, tools, topics, and languages, this is a MUST-HAVE book for artists interested in proficiency with the top technology available today Whether you're a programmer developing new animation functionality or an animator trying to get the most out of your current animation software, Computer Animation Complete: will help you work more efficiently and achieve better results. For programmers, this book provides a solid theoretical orientation and extensive practical instruction information you can put to work in any development or customization project. For animators, it provides crystal-clear guidance on determining which of your concepts can be realized using commercially available products, which demand custom programming, and what development strategies are likely to bring you the greatest success.
Expert instruction from a variety of pace-setting computer graphics researchers.Provides in-depth coverage of established and emerging animation algorithms.For readers who lack a strong scientific background, introduces the necessary concepts from mathematics, biology, and physics.A variety of individual languages and substances are addressed, but addressed separately - enhancing your grasp of the field as a whole while providing you with the ability to identify and implement solutions by category.

David Gould's acclaimed first book, Complete Maya Programming: An Extensive Guide to MEL and the C++ API, provides artists and programmers with a deep understanding of the way Maya works and how it can be enhanced and customized through programming. In his new book David offers a gentle, intuitive introduction to the core ideas of computer graphics.
Each concept is explained progressively and is fully implemented in both MEL and C++ so that an artist or programmer can use the source code directly in their own programs. Geometry and modeling are covered in detail with progressively more complex examples demonstrating all of Maya's possible programming features. David Gould's first volume is widely regarded as the most authoritative reference on Maya programming. Volume II continues this tradition and provides an unmatched guide for the artist and programmer tackling complex tasks.
* Covers a spectrum of topics in computer graphics including points and vectors, rotations, transformations, curves and surfaces (polygonal, NURBS, subdivision), and modeling.
* Offers insights to Maya's inner workings so that an artist or programmer can design and develop customized tools and solutions.
* Discusses problem solving with MEL (Maya's scripting language) and the more powerful and versatile C++ API, with plenty of code examples for each.

Learning Maya, the world's leading 3D animation and effects package, is a challenge, especially for those who want to master Maya's versatile programming features in addition to its built-in tools.
Finally, here is a practical, step-by-step guide that shows how to use Maya to its fullest potential, beginning with the basics. Readers of Complete Maya Programming will first gain a thorough understanding of Maya's inner workings, and then learn how to customize and extend Maya with scripts and plugins that take control and productivity to new levels.
Users new to programming can apply Maya's easy scripting language MEL (Maya Embedded Language), while more advanced users can work with the C++ API (Application Progamming Interface). Both a fundamental tutorial for Maya beginners and a solid reference for experienced developers, Complete Maya Programming is every user's guide to Maya mastery.
* Provides a multitude of real-world examples illustrating applications of Maya programming.
* Demonstrates how to use MEL to control Maya, customize its interface, automate procedures, and more
* Details how to use the C++ API to modify Maya functionality and develop tools and features to meet any need
* Explains when to use MEL, when to use the C++ API, and how to use them together
* Ideal for technical directors, developers, or anyone wishing to to master Maya
* Provides a storehouse of MEL scripts and C++ source code, glossary, and list of resources, available at www.davidgould.com

Charles Carter, dubbed Carter the Great by Houdini himself, was born into privilege but became a magician out of need: only when dazzling an audience can he defeat his fear of loneliness. But in 1920s America the stakes are growing higher, as technology and the cinema challenge the allure of magic and Carter's stunts become increasingly audacious. Until the night President Harding takes part in Carter's act only to die two hours later, and Carter finds himself pursued not only by the Secret Service but by a host of others desperate for the terrible secret they believe Harding confided in him. Seamlessly blending reality and fiction, Gold lays before us a glittering and romantic panorama of our modern world at a point of irrevocable change.

'I Will Be Complete is the best memoir I've read in years. It's likely the best memoir published in years.' Darin Strauss, author of Half a Life and Chang and Eng From the bestselling author of Carter Beats the Devil and Sunnyside, a shocking, big-hearted memoir about his bizarre upbringing in California in the 1970s and how he survived it. Glen David Gold grew up rich on the beaches of 1970s California, until his father lost a fortune and his parents divorced when he was ten. Glen and his English mother moved to San Francisco, where she was fleeced by a series of charming con men and turned increasingly wayward. When he was twelve, she took off for New York without telling him, leaving him to fend for himself. On midnight streets and at drug-fuelled parties, wise-cracking his way through an alarming adult world, Glen watched his mother's countless, wild attempts to reinvent herself. In this exceptional memoir, acclaimed novelist Glen David Gold captures his bizarre, lonely upbringing and how it shaped him as an adult with stunning insight and unsparing candour. Shocking, mordantly funny and achingly affecting, he tells an unforgettable story of the years he spent trying to rescue his mother - and his ultimate realisation that only by breaking free could he ever hope to be complete. 'The prose is crystalline, hard as real diamonds, flashing, revealing. The story is simple, just a boy and his mother's long disintegration, but the journey is darkly complicated, heartbreaking, beautiful as hell.' Mark Childress, author of Crazy in Alabama

From the author of CARTER BEATS THE DEVIL comes a panoramic tale of power and stardom, ambition and dreams that reaches from California in 1916 to the battlefields of France and the icy wastes of northern Russia. At the heart of its enthralling cast of characters - which includes a thieving Girl Scout, Mary Pickford, a charismatic British general and even the dog Rin Tin Tin - lies the troubled genius that was Charlie Chaplin. Here America debuts on the world stage in the Great War, Hollywood blossoms into a global phenomenon, and the cult of celebrity is born. Here, in a novel as darkly comic as it is thrilling, the modern age dawns.