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Ribonucleic acids are central to cellular and molecular processes
and perform vital functions in both structural and functional
roles. RNA molecules form the bridge between the stable genetic
information contained within DNA and enzymes and proteins that
carry out much of the metabolism within the cell. Many of the sites
of protein synthesis, the ribosomes within the cell, are composed
of these ribonucleic acids as are the tRNA molecules that deliver
the amino acid building blocks to the ribosomes. Of all the RNA
species, the nucleic acid intermediate, messenger RNA, is a
desirable source of material to biologists, since this reflects
much of, what ultimately, is translated into enzymes and proteins.
In order to determine the qualitative and quantitative changes in
mRNA expression, a vast number of molecular biological techniques
have been developed. Key molecular methods that provide the means
to initially isolate and analyze RNA molecules are the focus of
this volume. In putting together this collection of protocols, we
have tried to provide techniques that are most applicable and
widely used. In particular, there are a number of iso- tion
techniques included that have been developed, modified, or adapted
to enable extraction from a variety of cell types, organisms, or
subcellular organelles. Successful isolation of intact RNA is an
essential starting point for any sub- quent analysis. This is why
we have aimed to make this section comprehensive. The analysis of
RNA is the focus of the following chapters.
Ribonucleic acids are central to cellular and molecular processes
and perform vital functions in both structural and functional
roles. RNA molecules form the bridge between the stable genetic
information contained within DNA and enzymes and proteins that
carry out much of the metabolism within the cell. Many of the sites
of protein synthesis, the ribosomes within the cell, are composed
of these ribonucleic acids as are the tRNA molecules that deliver
the amino acid building blocks to the ribosomes. Of all the RNA
species, the nucleic acid intermediate, messenger RNA, is a
desirable source of material to biologists, since this reflects
much of, what ultimately, is translated into enzymes and proteins.
In order to determine the qualitative and quantitative changes in
mRNA expression, a vast number of molecular biological techniques
have been developed. Key molecular methods that provide the means
to initially isolate and analyze RNA molecules are the focus of
this volume. In putting together this collection of protocols, we
have tried to provide techniques that are most applicable and
widely used. In particular, there are a number of iso- tion
techniques included that have been developed, modified, or adapted
to enable extraction from a variety of cell types, organisms, or
subcellular organelles. Successful isolation of intact RNA is an
essential starting point for any sub- quent analysis. This is why
we have aimed to make this section comprehensive. The analysis of
RNA is the focus of the following chapters.
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