The many thousands of human-made and other chemical compounds present in the environment offer a serious challenge to our btosphere. It is appropriate, therefore, that our response to these products of human kno- edge and ingenuity should draw on a body of mtenstve scientific endeavor that is no less impressive. Bioremediation offers the possibility of harnessing the diversity of the biosphere to degrade, remove, alter, or otherwise detoxify these various chemicals. It brings together scientists from a wide variety of disciplines and backgrounds, such as microbiology, molecular biology, a- lytical chemistry, and chemical and environmental engineering, among o- ers. These different fields, each with its own individual approach, have actively contributed to the development of bioremediation research tn recent years. The prmcipal objective of Bioremediation Protocols is to make the fruits of some of this research available in a different format to that of the textbook or journal article. It provides a selection of clearly written laboratory pro- cols presented as stepwise, easy-to-follow mstructions. In common with p- wous volumes in this and the companion Methods in Molecular Biology series, an extensive "Notes" section is provided with each chapter. This contains u- ful mformation (of a type often not normally included m a research paper) supplementmg the protocol. Reviews and case studies are also included to provide a deeper context to the methods chapters.

The many thousands of human-made and other chemical compounds present in the environment offer a serious challenge to our btosphere. It is appropriate, therefore, that our response to these products of human kno- edge and ingenuity should draw on a body of mtenstve scientific endeavor that is no less impressive. Bioremediation offers the possibility of harnessing the diversity of the biosphere to degrade, remove, alter, or otherwise detoxify these various chemicals. It brings together scientists from a wide variety of disciplines and backgrounds, such as microbiology, molecular biology, a- lytical chemistry, and chemical and environmental engineering, among o- ers. These different fields, each with its own individual approach, have actively contributed to the development of bioremediation research tn recent years. The prmcipal objective of Bioremediation Protocols is to make the fruits of some of this research available in a different format to that of the textbook or journal article. It provides a selection of clearly written laboratory pro- cols presented as stepwise, easy-to-follow mstructions. In common with p- wous volumes in this and the companion Methods in Molecular Biology series, an extensive "Notes" section is provided with each chapter. This contains u- ful mformation (of a type often not normally included m a research paper) supplementmg the protocol. Reviews and case studies are also included to provide a deeper context to the methods chapters.

Anxiety is not always psychological but rather a disease that can now be controlled. The symptoms are devastating: sudden unprovoked attacks of anxiety-accompanied by skipping and racing of the heart, shortness of breath, lightheadedness, dizzy spells. Even worse is the fear that you are losing your mind. But here's the good news. The more unexpected and unprovoked the attacks or the symptoms, the more likely that the victim is suffering from a biologically beset disease. What's more, medical science can now overcome this disease in virtually all cases through a combination of drugs and behavior therapy. Dr. David V. Sheenan, formerly of the Havard Medical School, is now the Director of Research of the College of Medicine at the University of South Florida in Tampa. He draws upon recent research and his own pioneering breakthroughs in the treatment of anxiety to present in this book: -Quizzes that will help you determine whether your anxiety is caused by normal stress or is the result of a genetically influenced physical disorder. Seven progressive stages of the disease-and the four steps necessary for recovery. Detailed case histories of former anxiety victims who are now leading full and productive lives. Reassurance and guidance to family members and friends who want to help, but don't know how. This book offers new hope to millions of men and women across the country.