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The Plasmodium spp. parasite was identified as the causative agent
of malaria in 1880, and the mosquito was identified as the vector
in 1897. Despite subsequent efforts focused on the epidemiology,
cell biology, immunology, molecular biology, and clinical
manifestations of malaria and the Plasmodium parasite, there is
still no licensed vaccine for the prevention of malaria. Physical
barriers (bed nets, window screens) and chemical prevention methods
(insecticides and mosquito repellents) intended to interfere with
the transmission of the disease are not highly effective, and the
profile of resistance of the parasite to chemoprophylactic and
chemotherapeutic agents is increasing. The dawn of the new
millennium has seen a resurgence of interest in the disease by
government and philanthropic organizations, but we are still faced
with compl- ities of the parasite, the host, and the vector, and
the interactions among them. Malaria Methods and Protocols offers a
comprehensive collection of protocols describing conventional and
state-of-the-art techniques for the study of malaria, as well as
associated theory and potential problems, written by experts in the
field. The major themes reflected here include assessing the risk
of infection and severity of disease, laboratory models, diagnosis
and typing, molecular biology techniques, immunological techniques,
cell biology techniques, and field applications.
The Plasmodium spp. parasite was identified as the causative agent
of malaria in 1880, and the mosquito was identified as the vector
in 1897. Despite subsequent efforts focused on the epidemiology,
cell biology, immunology, molecular biology, and clinical
manifestations of malaria and the Plasmodium parasite, there is
still no licensed vaccine for the prevention of malaria. Physical
barriers (bed nets, window screens) and chemical prevention methods
(insecticides and mosquito repellents) intended to interfere with
the transmission of the disease are not highly effective, and the
profile of resistance of the parasite to chemoprophylactic and
chemotherapeutic agents is increasing. The dawn of the new
millennium has seen a resurgence of interest in the disease by
government and philanthropic organizations, but we are still faced
with compl- ities of the parasite, the host, and the vector, and
the interactions among them. Malaria Methods and Protocols offers a
comprehensive collection of protocols describing conventional and
state-of-the-art techniques for the study of malaria, as well as
associated theory and potential problems, written by experts in the
field. The major themes reflected here include assessing the risk
of infection and severity of disease, laboratory models, diagnosis
and typing, molecular biology techniques, immunological techniques,
cell biology techniques, and field applications.
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