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This volume expands upon the previous edition with current,
detailed protocols for investigating membranes and their component
lipids in artificial membranes, cells, and in silico. Chapters
focus on properties of the component lipids, membranes and their
biophysical properties, fluorescent probes for studying membranes,
sample preparation, physical techniques to study membrane
composition, properties , and function, behavior of cholesterol
within a bilayer and examination of cholesterol-dependent phase
separation. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
practical, Methods in Membrane Lipids, Second Edition seeks to aid
scientist in further study into membrane lipids.
This volume expands upon the previous edition with current,
detailed protocols for investigating membranes and their component
lipids in artificial membranes, cells, and in silico. Chapters
focus on properties of the component lipids, membranes and their
biophysical properties, fluorescent probes for studying membranes,
sample preparation, physical techniques to study membrane
composition, properties , and function, behavior of cholesterol
within a bilayer and examination of cholesterol-dependent phase
separation. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Authoritative and
practical, Methods in Membrane Lipids, Second Edition seeks to aid
scientist in further study into membrane lipids.
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